Caco-2 Engineered Control Cells

The C2BBe1 cells, a sublcone of Caco-2 cells, correspond to ATCC CRL-2101. The C2BBe1 cells are adenocarcinoma, epithelial cells from a human caucasian male (aged 72 years).

The three week production lead time begins on the Monday following a purchase, in the third week the plates are shipped on Tuesday for receipt on Wednesday or Thursday. As a biologic product that is shipped at room temperature the cells must be processed immediately upon receipt.

A minimum of 4 assay ready plates is required to purchase. Please allow for 1 week after ordering, for the plates to be seeded. Plates will be shipped to the customer on day 16 of the 21-day culturing process.

The Engineered Control cells can be used for drug permeability assays. They may also be used as the control in conjunction with C2BBe1 Transporter Knockout cell lines for drug/transporter interaction studies. Engineered control cells have been used in the in vitro Caco-2 permeability assay. The following posters and articles demonstrate how Caco-2 cells can be used for cell based assays:

Transporter Function in Caco-2 Cells with Targeted P-Glycoprotein, MRP2 and BCRP Gene Knockout Using Zinc Finger Nucleases

Comparison of Function and Relative Transporter Protein Concentrations in Caco-2 Cells with Single and Double Knockouts of the ABCB1, ABCG2, and ABCC2 Genes

Caco-2 Transporter Knockout Cell Based Assays

The Caco-2 subclone, C2BBe1 Engineered Control cells, are ideal for permeability and transporter analyses as they express multiple transporters, are human derived and grow in a homogenous monolayer that forms tight junctions which is necessary for efflux ratio analysis. Other benefits include:

• The 24-well Transwell format enables the these cells to be included in standard drug transporter protocols
• Human assay with no interference from animal inhibitors
• The 21-day cell culture period is reduced to 6-days for customers, saving valuable resources

Tested for Mycoplasma, bacterial and fungal content, post-freeze viability, short terminal repeat (STR) analysis for cell line identification.

This product is covered under a consumable purchase agreement for one-time use only. For more information:ADME/Tox Cell Lines License