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Merck
CN

650498

氯仿

HPLC Plus, for HPLC, GC, and residue analysis, ≥99.9%, contains amylenes as stabilizer

别名:

三氯甲烷

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关于此项目

经验公式(希尔记法):
CHCl3
化学文摘社编号:
分子量:
119.38
UNSPSC Code:
12190000
NACRES:
NA.04
PubChem Substance ID:
EC Number:
200-663-8
Beilstein/REAXYS Number:
1731042
MDL number:
Assay:
≥99.9%
Grade:
HPLC Plus
Technique(s):
HPLC: suitable, gas chromatography (GC): suitable
Bp:
60.5-61.5 °C (lit.)
Vapor pressure:
160 mmHg ( 20 °C)
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grade

HPLC Plus

Quality Level

vapor density

4.1 (vs air)

vapor pressure

160 mmHg ( 20 °C)

assay

≥99.9%

form

liquid

contains

amylenes as stabilizer

technique(s)

HPLC: suitable, gas chromatography (GC): suitable

impurities

≤0.02% water, ≤0.5 ppb Fluorescence (quinine) at 365 nm

evapn. residue

≤0.0001%

halogenated residue

<10 ng/L (as heptachlor epoxide)

refractive index

n20/D 1.445 (lit.)

bp

60.5-61.5 °C (lit.)

mp

−63 °C (lit.)

density

1.48 g/mL at 25 °C, 1.492 g/mL at 25 °C (lit.)

λ

H2O reference

UV absorption

λ: 245 nm Amax: 1.00, λ: 250 nm Amax: 0.30, λ: 275-400 nm Amax: 0.005

suitability

suitable for residue analysis

application(s)

food and beverages

SMILES string

ClC(Cl)Cl

InChI

1S/CHCl3/c2-1(3)4/h1H

InChI key

HEDRZPFGACZZDS-UHFFFAOYSA-N

General description

Chloroform is an organic chlorinated hydrocarbon (CHC) commonly used in industries as a solvent.

Application

Chloroform was used in the making of silanized coverslips for DNA combing.
It may be used as:
  • Extractant in solvent extraction process.
  • A component of the binary mobile phases for liquid chromatography–atmospheric pressure photoionization-mass spectrometry (LC–APPIMS) of triacylglycerol model compounds.
  • Solvent for recrystallization.
适用于高效液相色谱法、分光光度法、环境检测
Chloroform may be used in the following:

  • Extraction of active constituents of anti-inflammatory drugs for their analysis by attenuated total reflectance-Fourier transform infrared spectroscopic (ATR-FTIR) methods
  • Estimation of ascorbic acid, free phenolic acids, and flavonoids in extracts of chloroform, methanol, water and, acetic acid obtained from two edible leaf samples using reversed-phase high-performance liquid chromatography (RP-HPLC) coupled to photodiode array detector (DAD)
  • Preparation of cannabis product extracts for the analysis of delta-9-tetrahydrocannabinol (THC) residues on hands of suspected consumers by ion-mobility spectrometry (IMS)
  • Investigation of different solvents to measure their recovery ratios for the extraction of acrylamide and 5-hydroxymethylfurfural (HMF) from seafood samples to develop an HPLC-MS/MS-based method for their quantification
  • Extraction of pesticides by direct-immersion single drop micro-extraction (SDME) method from mango samples for their subsequent analysis by GC-MS
  • Sample preparation in the profiling of lipids from complex biological samples using off-line mixed-mode liquid chromatography coupled with reversed phase high performance liquid chromatography-high resolution mass spectrometry

Packaging

M-Bottle for Solvents
As a global leader in lab reagents, we are constantly looking for new ways to optimize the safety of our products. The newly developed 4L solvent bottle design features advanced sealing technology that eliminates leaks to make the handling of solvents safer and more convenient than ever before.
See all the new features here!

Other Notes

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pictograms

Skull and crossbonesHealth hazard

signalword

Danger

Hazard Classifications

Acute Tox. 3 Inhalation - Acute Tox. 4 Oral - Carc. 2 - Eye Irrit. 2 - Repr. 2 - Skin Irrit. 2 - STOT RE 1 Oral - STOT SE 3

target_organs

Central nervous system, Liver,Kidney

存储类别

6.1D - Non-combustible acute toxic Cat.3 / toxic hazardous materials or hazardous materials causing chronic effects

wgk

WGK 3

flash_point_f

does not flash

flash_point_c

does not flash

法规信息

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实验方案

A guide to create solvent systems used for the thin-layer chromatography assay of Novabiochem products.


Silanization of coverslips for DNA combing.
Labit H, et al.
Biotechniques, 11, 50-50 (2009)
Sheng-Suan Cai et al.
Journal of chromatography. A, 1173(1-2), 88-97 (2007-10-30)
In this work, we evaluate the performance of liquid chromatography-atmospheric pressure photoionization-mass spectrometry (LC-APPI-MS) for non-aqueous reversed phase analysis of six triacylglycerol model compounds using six binary mobile phases including MeOH/iPrOH, MeOH/CHCl(3), MeOH/CH(2)Cl(2), CH(3)CN/iPrOH, CH(3)CN/CHCl(3), and CH(3)CN/CH(2)Cl(2). All mobile phases
Electrogenerated chemiluminescent emission from an organized (L-B) monolayer of a Ru(bpy)32+-based surfactant on semiconductor and metal electrodes.
Zhang X and Bard AJ.
The Journal of Physical Chemistry, 92(20), 5566-5569 (1988)



全球贸易项目编号

货号GTIN
650498-4L04061837479588
650498-4X4L04061837479595
650498-1L04061837479564
650498-6X1L04061832732442