form
powder (lyophilized)
Quality Level
contains
60% lactose as stabilizer
technique(s)
affinity chromatography: suitable
capacity
≥1 μmol/mL, packed gel capacity (5′-ATP)(bound by a C6-spacer to C-8 of ATP)
storage temp.
−20°C
General description
The lactose-stabilizer must be removed prior to use by washing the gel onto filter with water or buffer.
Application
Adenosine 5′-triphosphate, immobilized on Agarose 4B (5′-ATP-agarose 4B) is intended for use in affinity chromatography. 5′-ATP-agarose 4B has been shown to bind enzymes with affinity to 5′-ATP. 5′-ATP-agarose 4B may be considered for used with enzymes that bind to other ATP-agarose and sepharose affinity media or beads.
Packaging
Bottomless glass bottle. Contents are inside inserted fused cone.
Other Notes
Purification of cofactor-dependent enzymes by affinity chromatography.
存储类别
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
Purification of cofactor-dependent enzymes by affinity chromatography.
Lee C-Y et al.
Analytical biochemistry, 77(1), 90-102 (1977-01-01)
ATP-and dATP-substituted agaroses and the purification of ribonucleotide reductases.
O Berglund et al.
Methods in enzymology, 34, 253-261 (1974-01-01)
G A Nevinsky et al.
Applied biochemistry and biotechnology, 75(1), 77-91 (1999-04-24)
This article presents evidence that protein kinase activity is an intrinsic property of secretory immunoglobulin A (sIgA) from milk of healthy human mothers. Polyclonal sIgA was purified by sequential chromatography on protein A-Sepharose, DEAE-cellulose, and gel filtration on Toyopearl HW-55
Cheng-Zhu Wu et al.
Archives of pharmacal research, 33(12), 1997-2001 (2010-12-31)
The molecular chaperone heat shock protein 90 (Hsp90) is responsible for maintaining the correct folding and stability of many signaling proteins. It is a promising target of cancer therapeutics and several other diseases, including neurodegenerative disease, nerve injuries, inflammation, and
Zhengyu Yin et al.
Biochemistry, 48(2), 336-345 (2008-12-31)
(-)-Epigallocatechin-3-gallate (EGCG), a major component of green tea, protects against certain types of cancers, although the mechanism has not yet been determined. It was previously demonstrated that EGCG blocks aryl hydrocarbon receptor (AhR)-mediated transcription induced by the potent carcinogen 2,3,7,8-tetrachlorodibenzo-p-dioxin
全球贸易项目编号
| 货号 | GTIN |
|---|---|
| 180262-1G | 04061825762876 |
| 180262-5G | 04061832098524 |
| 02065-25ML | 04061838219015 |
| 02065-1ML | 04061838612151 |
| 02065-5ML | 04061838219022 |
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