02065
Adenosine 5′-triphosphate, immobilized on Agarose 4B
suitable for affinity chromatography, powder (lyophilized)
别名:
5′-ATP-agarose 4B
form
powder (lyophilized)
contains
60% lactose as stabilizer
technique(s)
affinity chromatography: suitable
capacity
≥1 μmol/mL, packed gel capacity (5′-ATP)(bound by a C6-spacer to C-8 of ATP)
storage temp.
−20°C
Quality Level
General description
The lactose-stabilizer must be removed prior to use by washing the gel onto filter with water or buffer.
Application
Adenosine 5′-triphosphate, immobilized on Agarose 4B (5′-ATP-agarose 4B) is intended for use in affinity chromatography. 5′-ATP-agarose 4B has been shown to bind enzymes with affinity to 5′-ATP. 5′-ATP-agarose 4B may be considered for used with enzymes that bind to other ATP-agarose and sepharose affinity media or beads.
Packaging
Bottomless glass bottle. Contents are inside inserted fused cone.
Other Notes
Purification of cofactor-dependent enzymes by affinity chromatography.
存储类别
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
Cheng-Zhu Wu et al.
Archives of pharmacal research, 33(12), 1997-2001 (2010-12-31)
The molecular chaperone heat shock protein 90 (Hsp90) is responsible for maintaining the correct folding and stability of many signaling proteins. It is a promising target of cancer therapeutics and several other diseases, including neurodegenerative disease, nerve injuries, inflammation, and
ATP-and dATP-substituted agaroses and the purification of ribonucleotide reductases.
O Berglund et al.
Methods in enzymology, 34, 253-261 (1974-01-01)
G A Nevinsky et al.
Applied biochemistry and biotechnology, 75(1), 77-91 (1999-04-24)
This article presents evidence that protein kinase activity is an intrinsic property of secretory immunoglobulin A (sIgA) from milk of healthy human mothers. Polyclonal sIgA was purified by sequential chromatography on protein A-Sepharose, DEAE-cellulose, and gel filtration on Toyopearl HW-55
Purification of cofactor-dependent enzymes by affinity chromatography.
Lee C-Y et al.
Analytical biochemistry, 77(1), 90-102 (1977-01-01)
E Roggen et al.
European journal of biochemistry, 147(2), 225-232 (1985-03-01)
Poly(A) polymerase has been purified to near homogeneity from the cytoplasm of Artemia salina cryptobiotic gastrulae by ion-exchange chromatography on DEAE-cellulose, DEAE-Sepharose CL-6B and phosphocellulose P11, gel filtration on CL-Sepharose 6B, affinity chromatography on poly(A)-Sepharose 4B and ATP-agarose. The enzyme
我们的科学家团队拥有各种研究领域经验，包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.
联系客户支持