product line
BioReagent
Quality Segment
assay
≥90% (HPCE)
form
powder
manufacturer/tradename
ATTO-TEC GmbH
transmittance
254 nm, 655 nm
solubility
DMF: soluble, DMSO: soluble, acetonitrile: soluble
fluorescence
λex 655 nm; λem 680 nm in 0.1 M phosphate pH 7.0
λ
in ethanol (with 0.1% trifluoroacetic acid: 655 nm ± 3 nm)
suitability
suitable for fluorescence
storage temp.
−20°C
Application
Atto 655 conjugated to biotin is useful for binding to proteins (i.e., avidin and streptavidin). The fluorescent properties include excellent thermal and photo-stability, high quantum yield, strong absorption (663 nm) and fluorescence (684 nm), and low triplet formation. It is a zwitterionic dye, carrying a net electrical charge of zero. Electron donors such as guanine and tryptophan can easily quench the fluorescence of this fluorophore. Biotin conjugated with ATTO 655 was applied to assess efficiency of BSA/avidin structures bound to a surface.
Legal Information
This product is for Research use only. In case of intended commercialization, please contact the IP-holder (ATTO-TEC GmbH, Germany) for licensing.
存储类别
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)