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Merck
CN

12708

Anti-Mouse IgG - Mega 485

别名:

Mega 485-Anti-Mouse-IgG

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关于此项目

UNSPSC Code:
12352203
NACRES:
NA.46
MDL number:
Conjugate:
Mega 485 conjugate
Clone:
polyclonal
Application:
Citations:
3
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biological source

goat

conjugate

Mega 485 conjugate

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

contains

50% glycerol

species reactivity

mouse

concentration

1 mg/mL protein

fluorescence

λex 485 nm; λem 552 nm in PBS

storage temp.

−20°C

target post-translational modification

unmodified

General description

Anti-mouse IgG (whole molecule) is developed in goat using purified mouse IgG as the immunogen. Affinity isolated antigen specific antibody is purified from goat anti-mouse IgG antiserum to remove essentially all goat serum proteins, including immunoglobulins, which do not specifically bind to mouse IgG. The antibody preparation is solid phase adsorbed with human serum proteins to ensure minimal cross reactivity.
Immunoglobulin G (IgG) is a glycoprotein antibody that regulates immune responses such as phagocytosis and is also involved in the development of autoimmune diseases. Mouse IgGs have four distinct isotypes, namely, IgG1, IgG2a, IgG2b, and IgG3. IgG1 regulates complement fixation in mice.
Affinity isolated antigen specific antibody is purified from goat anti-mouse IgG antiserum to remove essentially all goat serum proteins, including immunoglobulins, which do not specifically bind to mouse IgG. The antibody preparation is solid phase adsorbed with human serum proteins to ensure minimal cross reactivity. Hence, Mega 485-goat anti-mouse IgG (whole molecule) is specific for mouse IgG.

Immunogen

purified mouse IgG

Application

Mega 485-goat anti-mouse IgG (whole molecule) can be used for fluorescence-based immunoassays.

Physical form

Mega 485-goat anti-mouse IgG (whole molecule) is provided in unit sizes of 1 ml as 1 mg/ml solutions in 0.1 M sodium phosphate, 0.1 M NaCl, pH 7.5, and has 5 mM sodium azide as a preservative.

Analysis Note

unconjugated dye ≤5% of total fluorescence

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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存储类别

12 - Non Combustible Liquids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves

法规信息

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历史批次信息供参考:

分析证书(COA)

Lot/Batch Number

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访问文档库

Zaklina Kovacevic et al.
The Journal of biological chemistry, 291(3), 1029-1052 (2015-11-05)
N-MYC downstream-regulated gene-1 (NDRG1) is a potent growth and metastasis suppressor that acts through its inhibitory effects on a wide variety of cellular signaling pathways, including the TGF-β pathway, protein kinase B (AKT)/PI3K pathway, RAS, etc. To investigate the hypothesis
Structure and function of human and murine receptors for IgG.
J C Unkeless et al.
Annual review of immunology, 6, 251-281 (1988-01-01)
Complement-fixing IgG1 constitutes a new subclass of mouse IgG.
P L Ey et al.
Nature, 281(5731), 492-493 (1979-10-11)

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