grade
Molecular Biology
product line
BioUltra
form
liquid
quality
2x concentrate
composition
BES, 10.7 g/L , NaCl, 16.0 g/L , Na2HPO4, 0.27 g/L
impurities
DNases, none detected, RNases, none detected, phosphatases, none detected, proteases, none detected
refractive index
n20/D 1.338
pH
7.1 (25 °C)
cation traces
Al: ≤1 mg/kg, Ba: ≤1 mg/kg, Bi: ≤1 mg/kg, Ca: ≤5 mg/kg, Cd: ≤1 mg/kg, Co: ≤1 mg/kg, Cr: ≤1 mg/kg, Cu: ≤1 mg/kg, Fe: ≤1 mg/kg, K: ≤20 mg/kg, Li: ≤1 mg/kg, Mg: ≤1 mg/kg, Mn: ≤1 mg/kg, Mo: ≤1 mg/kg, Ni: ≤1 mg/kg, Pb: ≤1 mg/kg, Sr: ≤1 mg/kg, Zn: ≤1 mg/kg
λ
neat
UV absorption
λ: 260 nm Amax: 0.07, λ: 280 nm Amax: 0.02
application(s)
diagnostic assay manufacturing
Application
BES缓冲盐水可用于研究分子生物学、DNA和RNA纯化、生物缓冲液和两性离子化合物。BES缓冲盐水已应用于一项研究,以确定如何提高转染效率,并随后对休眠状态的人胰管上皮细胞进行长期培养。
Preparation Note
浓度(H2O):10.7 g/l BES,16.0 g/l NaCl,0.27 g/l Na2HPO4
存储类别
12 - Non Combustible Liquids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves
J. Sambrook et al.
Molecular Cloning: A Laboratory Manual, B10 (1989)
R Jesenofsky et al.
Pancreas, 17(3), 262-265 (1998-10-27)
Preparation of pancreatic duct epithelial cells from adult organs is possible by limited digestion and outgrowth of cells. These primary cells are mitotically active for only a short period. Therefore transfection with SV40 large-T antigen is one method to obtain
Karine Laulagnier et al.
Cellular and molecular life sciences : CMLS, 75(4), 757-773 (2017-09-29)
Amyloid beta peptide (Aβ), the main component of senile plaques of Alzheimer's disease brains, is produced by sequential cleavage of amyloid precursor protein (APP) and of its C-terminal fragments (CTFs). An unanswered question is how amyloidogenic peptides spread throughout the
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