产品名称
Atto 700, BioReagent, suitable for fluorescence, ≥90.0% (HPCE)
product line
BioReagent
assay
≥90.0% (HPCE)
manufacturer/tradename
ATTO-TEC GmbH
transmittance
254 nm
700 nm
fluorescence
λex 681 nm; λem 714 nm in 0.1 M phosphate pH 7.0
λ
in ethanol (with 0.1% trifluoroacetic acid: 692 nm ± 3 nm)
suitability
suitable for fluorescence
storage temp.
−20°C
Quality Level
Application
Atto 700 belongs to a new generation of fluorescent labels. The dye is designed for application in the area of life science, e.g. labeling of DNA, RNA or proteins. Characteristic features of the label are strong absorption, high fluorescence quantum yield, excellent thermal and photo-stability, very good water solubility and very little triplet formation. Atto 700 is a zwitterionic dye with a net electrical charge of zero. The fluorescence is efficiently quenched by electron donors like guanine, tryptophan, etc.
Atto labels have rigid structures that do not show any cis-trans-isomerization. Thus these labels display exceptional intensity with minimal spectral shift on conjugation.
General description
Atto 700 is a new label with high molecular absorption (1.2 x 105) and quantum yield (0.25) as well as sufficient Stoke′s shift between excitation and emission maximum. Atto 700 is characterized by high photostability and thermal stability. It is also excellently soluble in water. Due to an insignificant triplet formation rate, it is well suited for single molecule detection applications.
Legal Information
This product is for Research use only. In case of intended commercialization, please contact the IP-holder (ATTO-TEC GmbH, Germany) for licensing.
存储类别
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
法规信息
新产品
此项目有
Multicolour single molecule imaging in cells with near infra-red dyes.
Tynan, C.J., et al.
PLoS ONE, 4, e36265-e36265 (2012)
Jan Vogelsang et al.
Photochemical & photobiological sciences : Official journal of the European Photochemistry Association and the European Society for Photobiology, 8(4), 486-496 (2009-04-02)
The role and interplay of triplet states and radical ion states in single-molecule fluorescence spectroscopy has recently been elaborated providing us with new insights into the photophysics and photobleaching pathways of fluorescent dyes. Adjustment of fluorophore redox properties in combination
Patricia Haus et al.
Journal of biomolecular screening, 16(10), 1206-1216 (2011-10-27)
Histone deacetylases (HDACs) are important epigenetic factors regulating a variety of vital cellular functions such as cell cycle progression, differentiation, cell migration, and apoptosis. Consequently, HDACs have emerged as promising targets for cancer therapy. The drugability of HDACs has been
Daniel Riester et al.
Bioorganic & medicinal chemistry letters, 19(13), 3651-3656 (2009-05-22)
Histone deacetylases reside among the most important and novel target classes in oncology. Selective lead structures are intensively developed to improve efficacy and reduce adverse effects. The common assays used so far to identify new lead structures suffer from many
Sebastian van de Linde et al.
Photochemical & photobiological sciences : Official journal of the European Photochemistry Association and the European Society for Photobiology, 8(4), 465-469 (2009-04-02)
We introduce a general approach for multicolor subdiffraction-resolution fluorescence imaging based on photoswitching of standard organic fluorophores. Photoswitching of ordinary fluorophores such as ATTO520, ATTO565, ATTO655, ATTO680, or ATTO700, i.e. the reversible transition from a fluorescent to a nonfluorescent state
商品
Fluorescence lifetime measurement is advantageous over intensity-based measurements. Applications include fluorescence lifetime assays, sensing and FLI.
荧光寿命测量与基于强度的测量相比是有优势的。其应用包括荧光寿命分析，感测和FLI。
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