36438
Fluorescein diacetate 6-isothiocyanate
≥90% (HPLC)
别名:
Diacetyl-6-FITC, Fluorescein-6-isothiocyanate diacetate
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关于此项目
经验公式（希尔记法）:
C25H15NO7S
化学文摘社编号:
分子量:
473.45
NACRES:
NA.32
PubChem Substance ID:
UNSPSC Code:
12352200
MDL number:
InChI key
ZMGWMLCTFALXEN-UHFFFAOYSA-N
SMILES string
CC(=O)Oc1ccc2c(Oc3cc(OC(C)=O)ccc3C24OC(=O)c5ccc(cc45)N=C=S)c1
InChI
1S/C25H15NO7S/c1-13(27)30-16-4-7-19-22(10-16)32-23-11-17(31-14(2)28)5-8-20(23)25(19)21-9-15(26-12-34)3-6-18(21)24(29)33-25/h3-11H,1-2H3
assay
≥90% (HPLC)
form
powder
fluorescence
λex 496 nm; λem 517 nm in 0.1 M Tris pH 8.0, esterase
Application
Fluorescein diacetate 6-isothiocyanate (diacetyl-6-FITC, F6ITC), a structural analogue of fluorescein reactive with secondary amines, is used as an isomeric haptenic probe.
存储类别
13 - Non Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
法规信息
新产品
此项目有
Analysis of 3-(2-(ethylamino)propyl)-1,2,3,4-tetrahydro-5H(1)benzopyrano (3,4-c)pyridin-5-one in plasma by liquid chromatographic column switching after derivatizing the secondary amine with fluorescein-6-isothiocyanate.
Reynolds DL, Pachla LA.
J. Pharm. Pharm. Sci., 74, 1091-1094 (1985)
C A Swindlehurst et al.
Biophysical journal, 59(3), 619-628 (1991-03-11)
Relative differences in the active site environment of a monoclonal antibody when covalently bound to two isomeric haptens were studied using fluorescence quenching and lifetime measurements. Murine monoclonal antibody 4-4-20, a well-characterized high affinity antifluorescein antibody, served as the model
K M Weidner et al.
Molecular immunology, 29(3), 303-312 (1992-03-11)
Immunizations of high affinity anti-fluorescein monoclonal antibody 4-4-20 affinity labeled with fluorescein 5-isothiocyanate into a rabbit elicited antibodies specific for the liganded conformation of 4-4-20 (termed "anti-metatype" antibodies). Reaction of liganded 4-4-20 with anti-metatype antibodies caused significant delay (up to
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