T-bet, a T-box transcription factor, is expressed in CD4+ T-lymphocytes committed to T-helper (T_h)1 T-cell development from naïve T-helper precursor cells (T_hp) and redirects T_h2 T-cells to T_h1 development. Anti-T-bet is a marker of mature T-cells and is expressed at very low levels in T_hp cells and is absent in precursor T-lymphoblastic leukemia/lymphoma cells. Scattered small lymphocytes in the interfollicular T-cell zone of reactive lymphoid tissue, including tonsil, lymph node, and spleen exhibited nuclear staining for anti-T-bet, with no anti-T-bet staining observed in germinal centers or mantle or marginal zones. T-bet is expressed in a significant subset of B-cell lymphoproliferative disorders, particularly at an early stage of B-cell development (precursor B-cell lymphoblastic leukemia/lymphoblastic lymphoma), and B-cell neoplasms derived from mature B-cells, including CLL/SLL, marginal zone lymphoma, and hairy cell leukemia. In contrast, B-cell neoplasms derived from pre-germinal center or germinal center B-cells, including mantle cell lymphoma, follicular lymphoma, diffuse large B-cell lymphoma, and Burkitt lymphoma are negative for anti-T-bet. Therefore, anti-T-bet should serve as a useful marker for the diagnosis and subtyping of B-cell and T-cell lymphoproliferative disorders.

T-bet Positive Control Slides, Product No. 368S, are available for immunohistochemistry (formalin-fixed, paraffin-embedded sections).

Solution in Tris Buffer, pH 7.3-7.7, with 1% BSA and <0.1% Sodium Azide

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