37294
Dihydrofolate Reductase from bovine liver
~8 U/mL, ammonium sulfate suspension, off-white
别名:
Tetrahydrofolate Dehydrogenase
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关于此项目
化学文摘社编号:
UNSPSC Code:
12352204
NACRES:
NA.54
MDL number:
Specific activity:
≥6 U/mg protein
Biological source:
bovine liver
Concentration:
~8 U/mL
biological source
bovine liver
form
ammonium sulfate suspension
specific activity
≥6 U/mg protein
concentration
~8 U/mL
color
off-white
density
1.2 g/mL at 20 °C
storage temp.
2-8°C
Application
Dihydrofolate reductase (DHFR) is involved in the synthesis of purines, thymidine and glycine in folate metabolism. DHFR, from bovine liver, is used for methotrexate analysis . DHFR is useful to study various cancers such as sarcomas, leukemias and head and neck cancers .
Biochem/physiol Actions
Dihydrofolate reductase (DHFR) reduces dihydrofolic acid to tetrahydrofolic acid, using NADPH as an electron donor. The binding of antitumor agents, such as methotrexate, to DHFR prevents the formation of reduced folates, which are essential for DNA synthesis .
Physical form
Suspension in 3.6 M ammonium sulfate solution, pH 7.0
Other Notes
1 U corresponds to the amount of enzyme which converts 1 μmol 7,8-dihydrofolate and NADPH to 5,6,7,8-tetrahydrofolate and NADP per minute at pH 6.5 and 25 °C
In folate metabolism for the synthesis of purines, thymidine and glycine
Sales restrictions may apply
存储类别
10 - Combustible liquids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves