biological source
rabbit
conjugate
unconjugated
antibody form
culture supernatant
antibody product type
primary antibodies
clone
EP287, monoclonal
description
For In Vitro Diagnostic Use in Select Regions (See Chart)
form
buffered aqueous solution
species reactivity
human
packaging
vial of 0.1 mL concentrate (388R-14), vial of 0.5 mL concentrate (388R-15), vial of 1.0 mL concentrate (388R-16), vial of 1.0 mL predilute ready-to-use (388R-17), vial of 7.0 mL predilute ready-to-use (388R-18)
manufacturer/tradename
Cell Marque®
concentration
0.1-0.5 μg/mL (predilute), 50-500 μg/mL (concentrate)
technique(s)
immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:100-1:500 (concentrated)
isotype
IgG
control
tonsil
shipped in
wet ice
storage temp.
2-8°C
visualization
cytoplasmic
Gene Information
human ... NES(10763)
General description
Nestin is a class VI intermediate filament (IF) protein. Nestin is expressed in neural progenitor cells during development of the central nervous system and peripheral nervous system. Cytoplasmic anti-nestin staining mostly occurs under pathological conditions. It has been reported that nestin expression is significantly increased in melanoma and correlated with more advanced stages of the disease. Nestin immunoreactivity is also reported in melanoma cells of HMB-45-negative amelanotic and melanotic, non-desmoplastic melanoma. Nestin is also useful in subclassifying breast carcinoma. Only cytoplasmic staining is considered positive, whereas any nuclear staining is considered as background artifact.
Physical form
Solution in Tris Buffer, pH 7.3-7.7, with 1% BSA and <0.1% Sodium Azide
Preparation Note
Download the IFU specific to your product lot and format
Note: This requires a keycode which can be found on your packaging or product label.
Download the latest released IFU
Note: This IFU may not apply to your specific product lot.
Note: This requires a keycode which can be found on your packaging or product label.
Download the latest released IFU
Note: This IFU may not apply to your specific product lot.
Analysis Note
Canada - IVD
European Union - IVD
Japan - RUO
United States - IVD
European Union - IVD
Japan - RUO
United States - IVD
Other Notes
For Technical Service please contact: 800-665-7284 or email: service@cellmarque.com
Legal Information
Cell Marque is a registered trademark of Merck KGaA, Darmstadt, Germany
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存储类别
12 - Non Combustible Liquids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
法规信息
监管及禁止进口产品
此项目有
Svetlana Brychtova et al.
Journal of cutaneous pathology, 34(5), 370-375 (2007-04-24)
Nestin is one of the intermediate filaments that are expressed in proliferating neural progenitor cells during development of the central nervous system (CNS) and peripheral nervous system. Postnatal re-expression of the protein occurs mainly under pathological conditions, including injury and
Hua Li et al.
Cancer research, 67(2), 501-510 (2007-01-20)
Transcriptional profiling has identified five breast cancer subtypes, of which the basal epithelial is most aggressive and correlates with poor prognosis. These tumors display a high degree of cellular heterogeneity and lack established molecular targets, such as estrogen receptor-alpha, progesterone
Maho Kanoh et al.
The Journal of dermatology, 37(6), 505-511 (2010-06-12)
Nestin, a marker of neural stem cells, is expressed in the stem cells of the mouse hair follicle. The nestin-expressing hair follicle stem cells can differentiate into neurons, glia, keratocytes, smooth muscle cells and melanocytes in vitro. These pluripotent nestin-expressing
全球贸易项目编号
| 货号 | GTIN |
|---|---|
| 388R-14-RUO | 04061837570957 |
| 388R-15-RUO | 04061837570971 |
| 388R-17 | 04061837571008 |
| 388R-15 | 04061837570964 |
| 388R-17-RUO | 04061837571015 |
| 388R-14 | 04061837570940 |
| 388R-16 | 04061837570988 |
| 388R-16-RUO | 04061837570995 |
| 388R-18 | 04061837571022 |
| 388R-18-RUO | 04061837571039 |