Anti-Rabbit IgG−Abberior^® STAR 635 antibody produced in goat

Abberior STAR 635 is a dye developed for STED and confocal imaging in the red spectral region. It features exceptional photostability, very high brightness and low background labeling. The dye works extremely well with Abberior Instruments STEDYCON and Expert Line microscopes and Leica STED microscopes. Abberior STAR 635 can substitute dyes like Atto^™ 647N, Alexa Fluor^™ 647, or Cy^®5. It can be excited with lasers at wavelength between 630 - 650 nm. For STED, a depletion wavelength around 750 - 800 nm is recommended. Best results are obtained with freshly prepared samples.


Photophysical properties (carboxylic acid):
Absorption Maximum, λ_ex [nm]: 635 (PBS pH 7.4), 635 (H₂O), 635 (MeOH)
Extinction Coefficient, ε_max [M^-1cm^-1]: 110 000 (PBS pH 7.4), 110 000 (H₂O), 115 000 (MeOH)
Correction Factor, CF₂₆₀ = ε₂₆₀/ε_max: 0,26 (PBS pH 7.4)
Correction Factor, CF₂₈₀ = ε₂₈₀/ε_max: 0,38 (PBS pH 7.4)
Fluorescence Maximum, λ_em [nm]: 655 (PBS pH 7.4), 655 (H₂O), 656 (MeOH)
Recommended STED Wavelength, λ [nm]: 750-800
Fluorescence Quantum Yield, λ: 0,88 (PBS pH 7.4)
Fluorescence Lifetime, τ [ns]: 2,8 (PBS pH 7.4)

Abberior STAR 635 particularly well suits the Abberior Instruments 2-color 775nm STED microscope and the Leica TCS STED system. It delivers high-resolution STED images with very low background signal due to its improved water solubility and exceptional brightness. The dye serves as an ideal partner for several 2-color STED packages.

Designed and tested for fluorescent super-resolution microscopy

May contain significant amounts of BSA as stabilizer
unconjugated dye ≤5% of total fluorescence

ALEXA FLUOR is a trademark of Life Technologies

Atto is a trademark of Atto-Tec GmbH

Cy is a registered trademark of Cytiva

abberior is a registered trademark of Abberior GmbH