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Merck
CN

46067

Sigma-Aldrich

溴化乙啶 溶液

suitable for fluorescence, ~1% in H2O

别名:

3,8-二氨基-5-乙基-6-苯基菲啶溴化物, EtBr, 胡米溴铵

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关于此项目

经验公式（希尔记法）:
C21H20BrN3
化学文摘社编号:
1239-45-8
分子量:
394.31
Beilstein:
3642536
MDL编号:
MFCD00011724
UNSPSC代码:
12161505
PubChem化学物质编号:
57650684
NACRES:
NA.32

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质量水平

100

浓度

~1% in H2O

荧光

λex 480 nm; λem 620 nm in H2O
λex 518 nm; λem 605 nm (bound to DNA)
λex 530 nm; λem 600 nm in 50 mM phosphate buffer pH 7.0 (upon binding to DNA)

适用性

suitable for fluorescence

储存温度

2-8°C

SMILES字符串

[Br-].CC[n+]1c(-c2ccccc2)c3cc(N)ccc3c4ccc(N)cc14

InChI

1S/C21H19N3.BrH/c1-2-24-20-13-16(23)9-11-18(20)17-10-8-15(22)12-19(17)21(24)14-6-4-3-5-7-14;/h3-13,23H,2,22H2,1H3;1H

InChI key

ZMMJGEGLRURXTF-UHFFFAOYSA-N

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应用

溴化乙啶 (EtBr) 为聚丙烯酰胺凝胶电泳或琼脂糖凝胶电泳中最常用的核酸染料。EtBr 在结合到双链 RNA 上时其荧光可提高 21 倍，在结合到双链 DNA 上可提高 25 倍，从而使得在低染料浓度 (10μg/ml) 下无需背景脱色。溴化乙啶已用于核酸的许多荧光分析。已表明它可结合到单链 DNA（虽然强度不高）和三链 DNA 上。由于 EtBr 能够结合到 DNA 上，因此它是 DNA 聚合酶的抑制剂。

生化/生理作用

溴化乙啶可插入双链 DNA 和 RNA 中，并作为移码诱变剂。它还可与吖啶橙结合用于区分存活的、凋亡的和坏死的细胞。

制备说明

对于在电泳后进行凝胶染色，应先用水将储存液样品稀释到 0.5μg/ml，再将凝胶孵育 15-30 分钟。通常无需脱色，但是如果必须降低背景色，则可在水中脱色 15 分钟。然后可在紫外灯箱（254nm 波长）中检测 DNA 条带。也可将溴化乙啶加到凝胶和电泳缓冲液（终浓度 0.5μg/ml）中，并在电泳后立即显影。

象形图

Skull and crossbonesHealth hazard
GHS06,GHS08

警示用语：

Danger

危险声明

H331,H341

危险分类

Acute Tox. 3 Inhalation - Muta. 2

储存分类代码

6.1D - Non-combustible acute toxic Cat.3 / toxic hazardous materials or hazardous materials causing chronic effects

WGK

nwg

闪点(°F)

Not applicable

闪点(°C)

Not applicable

个人防护装备

Eyeshields, Faceshields, Gloves, type ABEK (EN14387) respirator filter

历史批次信息供参考:

分析证书(COA)

Lot/Batch Number
BCCJ8584
BCCG3060
BCCC4195
BCBW2534
BCBQ5502V

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