50277
5,6-Diaminofluorescein diacetate
BioReagent, suitable for fluorescence, 98% (HPCE)
别名:
4,5-Diaminofluorescein diacetate, DAF-2 DA
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关于此项目
经验公式（希尔记法）:
C24H18N2O7
化学文摘社编号:
分子量:
446.41
NACRES:
NA.32
PubChem Substance ID:
UNSPSC Code:
12352200
MDL number:
InChI
1S/C24H18N2O7/c1-11(27)30-13-3-5-16-21(7-13)32-22-8-14(31-12(2)28)4-6-17(22)24(16)18-10-20(26)19(25)9-15(18)23(29)33-24/h3-10H,25-26H2,1-2H3
SMILES string
CC(=O)Oc1ccc2c(Oc3cc(OC(C)=O)ccc3C24OC(=O)c5cc(N)c(N)cc45)c1
InChI key
PTSUYDXEEKDBQU-UHFFFAOYSA-N
product line
BioReagent
assay
98% (HPCE)
fluorescence
λex 491 nm; λem 513 nm
suitability
suitable for fluorescence
Application
5,6-Diaminofluorescein diacetate (DAF-2 DA), a cell permeable analog of DAF-2, is used in intracellular fluorimetric detection of nitric oxide/NOS production.
存储类别
13 - Non Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
法规信息
新产品
此项目有
H Kojima et al.
Neuroreport, 9(15), 3345-3348 (1998-12-17)
The biological functions of nitric oxide in the neuronal system remain controversial. Using a novel fluorescence indicator, DAF-2 DA, for direct detection of NO, we examined both acute rat brain slices and organotypic culture of brain slices to ascertain NO
Andréa C Celotto et al.
European journal of pharmacology, 656(1-3), 88-93 (2011-02-09)
We investigated the mechanism by which extracellular acidification promotes relaxation in rat thoracic aorta. The relaxation response to HCl-induced extracellular acidification (7.4 to 6.5) was measured in aortic rings pre-contracted with phenylephrine (Phe, 10(-6) M) or KCl (45mM). The vascular
A C Celotto et al.
Nitric oxide : biology and chemistry, 23(4), 269-274 (2010-08-05)
To investigate the mechanism through which the extracellular alkalinization promotes relaxation in rat thoracic aorta. The relaxation response to NaOH-induced extracellular alkalinization (7.4-8.5) was measured in aortic rings pre-contracted with phenylephrine (Phe, 10(-6) M). The vascular reactivity experiments were performed
H Kojima et al.
Analytical chemistry, 70(13), 2446-2453 (1998-07-17)
Nitric oxide is a gaseous, free radical which plays a role as an intracellular second messenger and a diffusable intercellular messenger. To obtain direct evidence for NO functions in vivo, we have designed and synthesized diaminofluoresceins (DAFs) as novel fluorescent
Shoji Tsuji et al.
Journal of pediatric hematology/oncology, 34(7), 500-502 (2012-09-01)
Chronic granulomatous disease (CGD) is an inherited disorder in which phagocytic leukocytes fail to generate superoxide (O(2)(-)) and antimicrobial oxidants. Patients with CGD develop recurrent and often life-threatening infections due to catalase-positive microorganisms. We attempted to measure the production of
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