产品线
BioReagent
方案
≥90% (coupling to thiols)
表单
solid
制造商/商品名称
ATTO-TEC GmbH
技术
UV/Vis spectroscopy: suitable
适用性
corresponds for UV test
suitable for fluorescence
储存温度
−20°C
应用
Atto 612Q is a new quencher for fluorescence characterized by high molecular absorption (114.000). Atto 612Q is characterized by a high photostability and thermostability. It is slightly hydrophilic, with excellent solubility in polar solvents as DMF or DMSO. Maleimides are well suited for coupling to thiol groups. This is similar to iodacetamides, but maleimides do react more thiol selective. They do not show significant reaction with histidine or methionine. Hydrolysis of maleimides to a mixture of isomeric nonreactive maleamic acids can compete significantly with thiol modification, particularly above pH 8. Maleimides may be used for labeling of amines, which usually requires a higher pH than reaction of maleimides with thiols.
法律信息
This product is for Research use only. In case of intended commercialization, please contact the IP-holder (ATTO-TEC GmbH, Germany) for licensing.
储存分类代码
13 - Non Combustible Solids
WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
法规信息
新产品
此项目有
Michiko Kimoto et al.
Journal of the American Chemical Society, 132(43), 15418-15426 (2010-10-14)
In the development of orthogonal extra base pairs for expanding the genetic alphabet, we created novel, unnatural base pairs between fluorophore and quencher nucleobase analogues. We found that the nucleobase analogue, 2-nitropyrrole (denoted by Pn), and its 4-substitutions, such as
Pete Crisalli et al.
Bioconjugate chemistry, 23(9), 1969-1980 (2012-08-24)
We describe the synthesis and properties of new fluorescence quenchers containing aldehyde, hydrazine, and aminooxy groups, allowing convenient bioconjugation as oximes or hydrazones. Conjugation to oligonucleotides proceeded in high yield with aniline as catalyst. Kinetics studies of conjugation show that
Magdalena Swierczewska et al.
Physical chemistry chemical physics : PCCP, 13(21), 9929-9941 (2011-03-08)
Fluorescence-based assays and detection techniques are among the most highly sensitive and popular biological tests for researchers. To match the needs of research and the clinic, detection limits and specificities need to improve, however. One mechanism is to decrease non-specific
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