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56496

钙黄绿素-AM

Name: 钙黄绿素-AM
Brand: SIGMA

Small Package (20 X 50 μg ), ≥95.0% (HPLC)

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别名:

荧光素氨羧络合试剂, 钙黄绿素四乙酰氧基 O,O′-二乙酸酯, Calcein O,O′-diacetate tetrakis(acetoxymethyl) ester

经验公式（希尔记法）:

C₄₆H₄₆N₂O₂₃

CAS号:

148504-34-1

分子量:

994.86

MDL编号:

MFCD05861516

PubChem化学物质编号:

329758218

检测方案

≥95.0% (HPLC)

荧光

λ_ex 496 nm; λ_em 516 nm±5 nm in 0.1 M Tris pH 8.0, esterase; Ca²⁺

储存温度

−20°C

SMILES string

CC(=O)OCOC(=O)CN(CC(=O)OCOC(C)=O)Cc1c(OC(C)=O)ccc2c1Oc3c(CN(CC(=O)OCOC(C)=O)CC(=O)OCOC(C)=O)c(OC(C)=O)ccc3C24OC(=O)c5ccccc45

InChI

1S/C46H46N2O23/c1-25(49)60-21-64-39(55)17-47(18-40(56)65-22-61-26(2)50)15-32-37(68-29(5)53)13-11-35-43(32)70-44-33(16-48(19-41(57)66-23-62-27(3)51)20-42(58)67-24-63-28(4)52)38(69-30(6)54)14-12-36(44)46(35)34-10-8-7-9-31(34)45(59)71-46/h7-14H,15-24H2,1-6H3

InChI key

XKFSBWQWNMZWFA-UHFFFAOYSA-N

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一般描述

钙黄绿素-AM，也称钙黄绿素-乙酰甲酯，是一种不发射荧光的分子，在细胞内酯酶的作用下会转化为阴离子荧光形式。在实验过程中它可以使黏附细胞连续成像。钙黄绿素-AM是一种必不可少的染料，可在保持细胞膜完整的情况下将钙黄绿素引入活细胞。钙黄绿素-AM中发射荧光的部分位于细胞间隙，在以酯酶依赖型关系在细胞中捕获，之后显示出细胞中的细胞毒性活性。

应用

钙黄绿素-AM是一种细胞活力染料，也可作为多药外排性转运体（multidrug efflux transporter）的中性底物。在癌症研究中，它可用作多药耐药蛋白MRP的中性底物。用于测定人红细胞活力和老化的荧光测试法是以钙黄绿素-AM为探针。

钙黄绿素的非荧光细胞渗透衍生物，水解后发出荧光。用作多药耐药蛋白MRP的中性底物；用于肿瘤研究。

储存分类代码

11 - Combustible Solids

WGK

WGK 3

闪点(°F)

Not applicable

闪点(°C)

Not applicable

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Novel fluorescence assay using calcein-AM for the determination of human erythrocyte viability and aging.

Daniela Bratosin et al.

Cytometry. Part A : the journal of the International Society for Analytical Cytology, 66(1), 78-84 (2005-05-26)

A highly sensitive, fast, and simple flow cytometric assay to assess human red blood cell (RBCs) viability and aging is reported. The assay described in this report is based on the use of acetoxymethyl ester of calcein (calcein-AM), a fluorescein

Cytotoxic activity of calcein acetoxymethyl ester (Calcein/AM) on primary cultures of human haematological and solid tumours.

B Jonsson et al.

European journal of cancer (Oxford, England : 1990), 32A(5), 883-887 (1996-05-01)

The aim of this study was to determine the in vitro cytotoxicity of calcein acetoxymethyl ester (Calcein/AM) on primary cultures derived from solid and haematological human tumours. Calcein/AM is a fluorescent dye that localises intracellularly after esterase-dependent cellular trapping and

Calcein-AM is a detector of intracellular oxidative activity.

Jacopo Uggeri et al.

Histochemistry and cell biology, 122(5), 499-505 (2004-10-27)

Calcein-acetoxymethylester (calcein-AM) is a non-fluorescent, cell permeant compound, which is converted by intracellular esterases into calcein, an anionic fluorescent form. It is used in microscopy and fluorometry and provides both morphological and functional information of viable cells. In this study

Parguerenes: Marine red alga bromoditerpenes as inhibitors of P-glycoprotein (ABCB1) in multidrug resistant human cancer cells.

Xiao-Cong Huang et al.

Biochemical pharmacology, 85(9), 1257-1268 (2013-02-19)

High intrinsic or acquired expression of membrane spanning, adenosine triphosphate binding cassette (ABC) transporter proteins, such as P-glycoprotein (P-gp), in cancers represents a major impediment to chemotherapy, with accelerated drug efflux leading to multi-drug resistance (MDR). Although ABC transporter inhibitors

Graft of the gelatin sponge scaffold containing genetically-modified neural stem cells promotes cell differentiation, axon regeneration, and functional recovery in rat with spinal cord transection.

Bao-Ling Du et al.

Journal of biomedical materials research. Part A, 103(4), 1533-1545 (2014-07-22)

Biological materials combined with genetically-modified neural stem cells (NSCs) are candidate therapy targeting spinal cord injury (SCI). Based on our previous studies, here we performed gelatin sponge (GS) scaffold seeded with neurotrophin-3 (NT-3) and its receptor TrkC gene modifying NSCs

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