生物来源
human dermis (fibroblast)
质量水平
重编程方法
retrovirus
描述
age (45-49)
制造商/商品名称
EBiSC™
性别
female
生长模式
adherent (pluripotent)
技术
cell culture | stem cell: suitable
相关疾病
ventricular tachycardia
运输
dry ice
储存温度
−196°C
一般描述
Induced pluripotent stem cells (iPSCs) are adult cells that have been reprogrammed to an embryonic stem cell–like state. The cells can replicate indefinitely or, under controlled conditions, can be differentiated into any other cell type such as nerve, heart or liver cells. Medical researchers are able to use iPS cells to test how different patients might respond to new drugs or to analyse how genetic diseases develop.
The EBiSC stem cell bank is a collection of human iPS cells available to academic and commercial researchers for use in disease modelling and other forms of stem cell research. The initial collection has been generated from a wide range of donors representing specific disease backgrounds and healthy controls. EBiSC has established many routine procedures for collecting, expanding and characterizing human iPS cell lines. The stem cell bank includes iPSC cell lines derived from neurodegenerative diseases (Alzheimer′s Disease, Parkinson′s Disease, Dementia, Motor Neuron Disease (ALS) - and Huntington′s Disease), eye and heart diseases, and lines from healthy control donors for age and sex matching.
The EBiSC stem cell bank is a collection of human iPS cells available to academic and commercial researchers for use in disease modelling and other forms of stem cell research. The initial collection has been generated from a wide range of donors representing specific disease backgrounds and healthy controls. EBiSC has established many routine procedures for collecting, expanding and characterizing human iPS cell lines. The stem cell bank includes iPSC cell lines derived from neurodegenerative diseases (Alzheimer′s Disease, Parkinson′s Disease, Dementia, Motor Neuron Disease (ALS) - and Huntington′s Disease), eye and heart diseases, and lines from healthy control donors for age and sex matching.
生化/生理作用
Depositor
Klinikum der Universität zu Köln
Klinikum der Universität zu Köln
Derivation
Primary cell type: Fibroblast of dermis
Primary cell developmental stage: Adult
Location of primary tissue procurement: Berlin, Germany
Tissue collection date: June 15, 2009
Passage number reprogrammed: P3
Reprogramming method
Vector type: Integrating
Vector: Virus
Virus type: Retrovirus
Gene list:
KLF4
MYC
POU5F1
SOX2
Have the reprogramming vectors been silenced: Unknown
Vector map: File
Other:From the same donor: UKKi007-A
Characterization
Analysis of Undifferentiated Cells
Marker expression: POU5F1 (OCT-4)(+)TRA1-80(+)SSEA-4(+)NANOG(+)
Differentiation potency
Ectoderm: In vitro spontaneous differentiation
Marker Expressed:B-TUBULIN(+)FOXG1(-)HES5(+)NEUROD1(+)PAX6 (+)Sox1(+)
Endoderm: In vitro spontaneous differentiation
Marker Expressed:BMP4(+)DCN(+)GATA4(-)HAND1(+)PDGF(+)PECAM (+)VIMENTIN(+)
Mesoderm: In vitro spontaneous differentiation
Marker Expressed:ACTN2, cardiac alpha actinin(+)CXCR4(+)FOXA2(-)GATA6 (+)GSC (+)MLC2v, myosin light chain 2v (+)MYH6, alpha myosin heavy chain(+)PITX1 (+)RYR2 (+)SOX17(+)
Microbiology / Virus Screening
HIV 1: Negative
HIV 2: Not done
Hepatitis B: Negative
Hepatitis C: Negative
Mycoplasma: Negative
Sterility
Inoculation for microbiological growth: No Contaminants Detected
Mycoplasma: Not Detected
Viability: Viable post-cryopreservation
Genotyping
Karyotyping
Karyotyping method: Molecular karyotyping using using OmniExpress Exome Chip
Genotyping
Genome-wide analysis:
SNP-genotyping using OmniExpress Exome Chip
STR/Fingerprinting: A 16 allele profile has been recorded and data is available upon request, after cell line purchase.
Primary cell type: Fibroblast of dermis
Primary cell developmental stage: Adult
Location of primary tissue procurement: Berlin, Germany
Tissue collection date: June 15, 2009
Passage number reprogrammed: P3
Reprogramming method
Vector type: Integrating
Vector: Virus
Virus type: Retrovirus
Gene list:
KLF4
MYC
POU5F1
SOX2
Have the reprogramming vectors been silenced: Unknown
Vector map: File
Other:From the same donor: UKKi007-A
Characterization
Analysis of Undifferentiated Cells
Marker expression: POU5F1 (OCT-4)(+)TRA1-80(+)SSEA-4(+)NANOG(+)
Differentiation potency
Ectoderm: In vitro spontaneous differentiation
Marker Expressed:B-TUBULIN(+)FOXG1(-)HES5(+)NEUROD1(+)PAX6 (+)Sox1(+)
Endoderm: In vitro spontaneous differentiation
Marker Expressed:BMP4(+)DCN(+)GATA4(-)HAND1(+)PDGF(+)PECAM (+)VIMENTIN(+)
Mesoderm: In vitro spontaneous differentiation
Marker Expressed:ACTN2, cardiac alpha actinin(+)CXCR4(+)FOXA2(-)GATA6 (+)GSC (+)MLC2v, myosin light chain 2v (+)MYH6, alpha myosin heavy chain(+)PITX1 (+)RYR2 (+)SOX17(+)
Microbiology / Virus Screening
HIV 1: Negative
HIV 2: Not done
Hepatitis B: Negative
Hepatitis C: Negative
Mycoplasma: Negative
Sterility
Inoculation for microbiological growth: No Contaminants Detected
Mycoplasma: Not Detected
Viability: Viable post-cryopreservation
Genotyping
Karyotyping
Karyotyping method: Molecular karyotyping using using OmniExpress Exome Chip
Genotyping
Genome-wide analysis:
SNP-genotyping using OmniExpress Exome Chip
STR/Fingerprinting: A 16 allele profile has been recorded and data is available upon request, after cell line purchase.
制备说明
Medium: Essential E8®
Passage method: EDTA
Matrix: Vitronectin
CO2 concentration: 5%
O2 concentration: 21%
Temperature: 37°C
Passage method: EDTA
Matrix: Vitronectin
CO2 concentration: 5%
O2 concentration: 21%
Temperature: 37°C
其他说明
Note: EAUA and CLIP must be completed before order fulfillment
法律信息
E8 is a registered trademark of WIsconsin Alumni Research Foundation non-stock Corporation
EBiSC is a trademark of Fraunhofer-Gesellschaft
储存分类代码
10 - Combustible liquids
WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
法规信息
高风险级别生物产品--人源产品
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