UKKi006-A

Induced pluripotent stem cells (iPSCs) are adult cells that have been reprogrammed to an embryonic stem cell–like state. The cells can replicate indefinitely or, under controlled conditions, can be differentiated into any other cell type such as nerve, heart or liver cells. Medical researchers are able to use iPS cells to test how different patients might respond to new drugs or to analyse how genetic diseases develop.

The EBiSC stem cell bank is a collection of human iPS cells available to academic and commercial researchers for use in disease modelling and other forms of stem cell research. The initial collection has been generated from a wide range of donors representing specific disease backgrounds and healthy controls. EBiSC has established many routine procedures for collecting, expanding and characterizing human iPS cell lines. The stem cell bank includes iPSC cell lines derived from neurodegenerative diseases (Alzheimer′s Disease, Parkinson′s Disease, Dementia, Motor Neuron Disease (ALS) - and Huntington′s Disease), eye and heart diseases, and lines from healthy control donors for age and sex matching.

Depositor
Klinikum der Universität zu Köln

Derivation
Primary cell type: Mesenchymal stem cell of the bone marrow
Primary cell developmental stage: Adult
Location of primary tissue procurement: Aachen, Germany
Tissue collection date: April 21, 2010
Passage number reprogrammed: P4
Reprogramming method
Vector type: Integrating
Vector: Virus
Virus type: Retrovirus
Gene list:
KLF4
MYC
POU5F1
SOX2
Have the reprogramming vectors been silenced: Yes
Merthods used: rtpcr
Notes on reprogramming vector silencing: Retrovirally encoded reprogramming factors OSKM are not expressed in iPSCs as determined by RT-PCR
Files showing reprogramming vector expressed or silenced: Image / Result
Vector map: File

Characterization
Analysis of Undifferentiated Cells
Marker expression: TRA1-80(+)ZFP42 (REX-1)(+)TRA 1-60 (+) SSEA-4(+)TRA 1-80(+)NANOG(+)POU5F1 (OCT-4)(+)

Microbiology / Virus Screening
HIV 1: Negative
HIV 2: Not done
Hepatitis B: Negative
Hepatitis C: Negative
Mycoplasma: Negative
Sterility
Inoculation for microbiological growth: No Contaminants Detected
Mycoplasma: Not Detected
Viability: Viable post-cryopreservation

Genotyping
Karyotyping
Cell line karyotype: Deletion of the short arm and duplikation of the long arm of Chr20 were detected.
Karyotyping method: Molecular karyotyping by SNP array
Genotyping
Genome-wide analysis:
Methylation profiling
STR/Fingerprinting: A 16 allele profile has been recorded and data is available upon request, after cell line purchase.

Medium: Essential E8^®
Passage method: EDTA
Matrix: Vitronectin
CO₂ concentration: 5%
O₂ concentration: 21%
Temperature: 37°C

Note: EAUA and CLIP must be completed before order fulfillment

E8 is a registered trademark of WIsconsin Alumni Research Foundation non-stock Corporation

EBiSC is a trademark of Fraunhofer-Gesellschaft