SIGi001-A-2

Induced pluripotent stem cells (iPSCs) are adult cells that have been reprogrammed to an embryonic stem cell–like state. The cells can replicate indefinitely or, under controlled conditions, can be differentiated into any other cell type such as nerve, heart or liver cells. Medical researchers are able to use iPS cells to test how different patients might respond to new drugs or to analyse how genetic diseases develop.

The EBiSC stem cell bank is a collection of human iPS cells available to academic and commercial researchers for use in disease modelling and other forms of stem cell research. The initial collection has been generated from a wide range of donors representing specific disease backgrounds and healthy controls. EBiSC has established many routine procedures for collecting, expanding and characterizing human iPS cell lines. The stem cell bank includes iPSC cell lines derived from neurodegenerative diseases (Alzheimer′s Disease, Parkinson′s Disease, Dementia, Motor Neuron Disease (ALS) - and Huntington′s Disease), eye and heart diseases, and lines from healthy control donors for age and sex matching.

Depositor
Sigma-Aldrich

Derivation
Primary cell type: Epithelium
Reprogramming method
Vector type: Integrating
Vector: Virus
Virus type: Retrovirus
Gene list:
KLF4
MYC
POU5F1
SOX2
Have the reprogramming vectors been silenced: Unknown

Characterization
Analysis of Undifferentiated Cells
Marker expression: POU5F1 (OCT-4)(+)TRA 1-60(+)SSEA-4(+)SSEA-1 (-)
Differentiation potency
Ectoderm: In vitro spontaneous differentiation
Marker Expressed:NEUROD(+)PAX6 (+)
Endoderm: In vitro spontaneous differentiation
Marker Expressed:GATA6(+)TBX5(+)
Mesoderm: In vitro spontaneous differentiation
Marker Expressed:AFP (+)SERPINA(+)

Microbiology / Virus Screening
HIV 1: Negative
HIV 2: Negative
Hepatitis B: Negative
Hepatitis C: Negative
Mycoplasma: Negative
Sterility
Inoculation for microbiological growth: No Contaminants Detected
Mycoplasma: Not Detected
Viability: Viable post-cryopreservation

Genotyping
Karyotyping
Passage number: 31
Cell line karyotype: No autosomal or sex chromosome aneuploidies detected
Karyotyping method: Karyolite BOBs
Genotyping
STR/Fingerprinting: A 16 allele profile has been recorded and data is available upon request, after cell line purchase.

Genetic Modification
Modifications not associated with diseases
Type of modification: Gene knock-in
Gene (target): SLC17A7
Chromosome location: 19q13.33
Transgene: T2A-GFP cassette
Delivery method: Zinc finger nuclease
Description: Induced pluripotent stem cell line with insertion of T2A-GFP cassette into SLC17a7 gene introduced by zinc finger nuclease (ZFN) technology.

Medium: mTeSR^®
Passage method: EDTA
Matrix: Matrigel^® / Geltrex^®
CO₂ concentration: 5%
O₂ concentration: 21%
Temperature: 37°C

Note: EAUA and CLIP must be completed before order fulfillment

EBiSC is a trademark of Fraunhofer-Gesellschaft

GELTREX is a registered trademark of Life Technologies Corporation

Matrigel is a registered trademark of Corning, Inc.

mTeSR is a registered trademark of WiCell Research Institute, Inc.