UOXFi005-C

Induced pluripotent stem cells (iPSCs) are adult cells that have been reprogrammed to an embryonic stem cell–like state. The cells can replicate indefinitely or, under controlled conditions, can be differentiated into any other cell type such as nerve, heart or liver cells. Medical researchers are able to use iPS cells to test how different patients might respond to new drugs or to analyse how genetic diseases develop.

The EBiSC stem cell bank is a collection of human iPS cells available to academic and commercial researchers for use in disease modelling and other forms of stem cell research. The initial collection has been generated from a wide range of donors representing specific disease backgrounds and healthy controls. EBiSC has established many routine procedures for collecting, expanding and characterizing human iPS cell lines. The stem cell bank includes iPSC cell lines derived from neurodegenerative diseases (Alzheimer′s Disease, Parkinson′s Disease, Dementia, Motor Neuron Disease (ALS) - and Huntington′s Disease), eye and heart diseases, and lines from healthy control donors for age and sex matching.

Depositor
University of Oxford

Derivation
Primary cell type: Fibroblast of dermis
Tissue collection date: April 4, 2011
Passage number reprogrammed: 5
Reprogramming method
Vector type: Integrating
Vector: Virus
Virus type: Retrovirus
Gene list:
KLF4
MYC
NANOG
POU5F1
SOX2
Absence of reprogramming vector(s): yes
Have the reprogramming vectors been silenced: Unknown
Other:From the same donor: UOXFi005-A, UOXFi005-B

Characterization
Analysis of Undifferentiated Cells
Marker expression: TRA 1-60(+)
PluriTest: Pluripotency score: 24.81
Novelty score: 1.54
Report: UOXFi005-C.PNG

Microbiology / Virus Screening
HIV 1: -
HIV 2: -
Hepatitis B: -
Hepatitis C: -
Mycoplasma: -

Genotyping
Karyotyping
Passage number: 15
Cell line karyotype: 46, XY
Karyotyping method: Illumina Human CytoSNP-12v2.1 bead chip array

Surface coating: Matrigel^®/Geltrex^®
Passage method: Enzyme-free cell dissociation / EDTA
O₂ concentration: 20%
CO₂ concentration: 5%
Culture medium: mTeSR^® 1
Passage Number banked (pre-EBiSC): 9
No. Vials banked (pre-EBiSC): 30

Note: EAUA and CLIP must be completed before order fulfillment

EBiSC is a trademark of Fraunhofer-Gesellschaft

GELTREX is a registered trademark of Life Technologies Corporation

Matrigel is a registered trademark of Corning, Inc.

mTeSR is a registered trademark of WiCell Research Institute, Inc.