UKKi021-B

Induced pluripotent stem cells (iPSCs) are adult cells that have been reprogrammed to an embryonic stem cell–like state. The cells can replicate indefinitely or, under controlled conditions, can be differentiated into any other cell type such as nerve, heart or liver cells. Medical researchers are able to use iPS cells to test how different patients might respond to new drugs or to analyse how genetic diseases develop.

The EBiSC stem cell bank is a collection of human iPS cells available to academic and commercial researchers for use in disease modelling and other forms of stem cell research. The initial collection has been generated from a wide range of donors representing specific disease backgrounds and healthy controls. EBiSC has established many routine procedures for collecting, expanding and characterizing human iPS cell lines. The stem cell bank includes iPSC cell lines derived from neurodegenerative diseases (Alzheimer′s Disease, Parkinson′s Disease, Dementia, Motor Neuron Disease (ALS) - and Huntington′s Disease), eye and heart diseases, and lines from healthy control donors for age and sex matching.

Depositor
Klinikum der Universität zu Köln

Derivation
Primary cell type: Fibroblast of dermis
Location of primary tissue procurement: Zone of skin
Tissue collection year: 2016
Passage number reprogrammed: 3
Reprogramming method
Vector type: Non-integrating
Vector: Episomal
Is the reprogramming vector detectable: No
Merthods used: pcr
Notes on reprogramming vector detection: episomal vector cassette absent
Files showing reprogramming vector expressed or silenced: Image / Result

Xeno free conditions: no
Derived under gmp: no
Available as clinical grade: no

Characterization
Analysis of Undifferentiated Cells
Marker expression:
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
CD30 Yes -
TRA 1-80 Yes - -
DNMT3b Yes -
CD90 Yes -
SSEA-4 Yes - -
SSEA-1 Yes -
NANOG Yes - -
OCT 4 Yes - -
FOXD3 Yes -
SOX2 Yes -
EpiPluriScore:
Score: 0,24
Marker Present Absent
mCpG
OCT4

Report:
Epi_Pluri_score_2017.jpg
Epi_pluri_score_result_20170621.pdf - Analysis Report Epi Pluri Score
Morphology:

hiPS NP0105-19 before banking and after thawing

hiPS NP0105-19 on different days post thaw
Differentiation potency
Ectoderm:
Ectoderm
In vitro directed differentiation
Marker Expressed
Nestin No
Endoderm:
Endoderm
In vitro directed differentiation
Marker Expressed
SOX17 Yes
Morphology:
Mesoderm:
Mesoderm
In vitro directed differentiation
Marker Expressed
BRACHYURY Yes
Morphology:
Microbiology / Virus Screening
HIV 1: Negative
HIV 2: Negative
Hepatitis B: Negative
Hepatitis C: Negative
Mycoplasma: Negative

Genotyping
Karyotyping
Passage number: 15
Cell line karyotype: Chr07: Larger duplication (20Mbp) mosaic (80%) on long arm.
Karyotyping method: Molecular karyotyping by SNP array
Image / Result

Surface coating: Vitronectin
Passage method: Enzyme-free cell dissociation / EDTA
O₂ concentration: 20%
CO₂ concentration: 5%
Culture medium: other
Culture base medium: StemFlex^™ Basal Medium
Main protein source: -
Passage Number banked (pre-EBiSC): 21
No. Vials banked (pre-EBiSC): 25

Note: EAUA and CLIP must be completed before order fulfillment

EBiSC is a trademark of Fraunhofer-Gesellschaft

StemFlex is a trademark of Life Technologies Corporation