69898
溴乙烷
suitable for fluorescence, BioReagent, ≥95% (HPCE)
别名:
单溴代二苯甲烷
登录查看公司和协议定价
关于此项目
经验公式（希尔记法）:
C10H11BrN2O2
CAS Number:
分子量:
271.11
Beilstein:
4430959
MDL编号:
UNSPSC代码:
12171500
PubChem化学物质编号:
NACRES:
NA.32
产品线
BioReagent
质量水平
方案
≥95% (HPCE)
表单
solid
mp
152-154 °C (lit.)
溶解性
DMF: soluble
DMSO: soluble
acetonitrile: soluble
methanol: soluble
荧光
λex 390 nm; λem 478 nm in 0.1 M phosphate pH 7.5 (after derivatization with glutathione)
λex 398 nm
适用性
suitable for fluorescence
储存温度
2-8°C
SMILES字符串
CC1=C(C)C(=O)N2N1C(CBr)=C(C)C2=O
InChI
1S/C10H11BrN2O2/c1-5-7(3)12-8(4-11)6(2)10(15)13(12)9(5)14/h4H2,1-3H3
InChI key
AHEWZZJEDQVLOP-UHFFFAOYSA-N
正在寻找类似产品? 访问 产品对比指南
一般描述
溴乙烷也被称为硫醇探针，是一种通过光解反应激活的荧光试剂。溴乙烷在溶液中与小硫醇基团（比如 GSH）和有反应活性的蛋白硫醇基团（如 血红蛋白）反应。 溴乙烷与硫醇的反应是二级反应，和pH相关。并且，通过与硫醇反应，激活了用于检测的水溶性荧光物质。
应用
溴乙烷被用于通过HPLC测定硫醇。 它适用于作为荧光测定2,3-二巯基丙烷-1-磺酸和其他二硫醇的柱前衍生试剂。 溴乙烷被用做研究牛心脏线粒体中对寡霉素敏感的ATP酶的荧光标记。
特点和优势
用溴乙烷分析低分子量硫醇的好处在于： 高选择性。
- 反应快速。
- 易于通过反相HPLC分离衍生物。
- 能穿透细胞。
包装
无底玻璃瓶。内含物在插入的融合锥体内。
其他说明
荧光硫醇特异性试剂
储存分类代码
11 - Combustible Solids
WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
个人防护装备
Eyeshields, Gloves, type N95 (US)
R M Maiorino et al.
Journal of chromatography, 374(2), 297-310 (1986-01-24)
The increasing therapeutic use of dithiol metal binding agents, such as 2,3-dimercaptopropane-1-sulfonic acid (DMPS), has stimulated the need for a sensitive and selective method for their determination in biological fluids. A method has now been developed in which DMPS was
P C Chinn et al.
Analytical biochemistry, 159(1), 143-149 (1986-11-15)
A highly sensitive and specific assay for Escherichia coli thioredoxin was developed using the thiol-specific reagent monobromobimane. Treatment of dithiothreitol-reduced thioredoxin with an excess of monobromobimane in Tris buffer (pH 8.0, 23 degrees C) for 30 min resulted in the
Determination of low-molecular-weight thiols using monobromobimane fluorescent labeling and high-performance liquid chromatography.
R C Fahey et al.
Methods in enzymology, 143, 85-96 (1987-01-01)
Carl Simonsson et al.
The Journal of investigative dermatology, 131(7), 1486-1493 (2011-01-14)
Allergic contact dermatitis (ACD) is the most prevalent form of human immunotoxicity. It is caused by skin exposure to haptens, i.e., protein-reactive, low-molecular-weight chemical compounds, which form hapten-protein complexes (HPCs) in the skin, triggering the immune system. These immunogenic HPCs
Michael Hall et al.
Proteomics, 10(5), 987-1001 (2010-01-06)
The light-dependent regulation of stromal enzymes by thioredoxin (Trx)-catalysed disulphide/dithiol exchange is known as a classical mechanism for control of chloroplast metabolism. Recent proteome studies show that Trx targets are present not only in the stroma but in all chloroplast
我们的科学家团队拥有各种研究领域经验，包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.
联系客户支持