69898
溴乙烷
suitable for fluorescence, BioReagent, ≥95% (HPCE)
别名:
单溴代二苯甲烷
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关于此项目
经验公式（希尔记法）:
C10H11BrN2O2
化学文摘社编号:
分子量:
271.11
UNSPSC Code:
12171500
NACRES:
NA.32
PubChem Substance ID:
MDL number:
Beilstein/REAXYS Number:
4430959
InChI
1S/C10H11BrN2O2/c1-5-7(3)12-8(4-11)6(2)10(15)13(12)9(5)14/h4H2,1-3H3
SMILES string
CC1=C(C)C(=O)N2N1C(CBr)=C(C)C2=O
InChI key
AHEWZZJEDQVLOP-UHFFFAOYSA-N
product line
BioReagent
assay
≥95% (HPCE)
form
solid
mp
152-154 °C (lit.)
solubility
DMF: soluble, DMSO: soluble, acetonitrile: soluble, methanol: soluble
fluorescence
λex 390 nm; λem 478 nm in 0.1 M phosphate pH 7.5 (after derivatization with glutathione), λex 398 nm
suitability
suitable for fluorescence
storage temp.
2-8°C
Quality Level
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General description
溴乙烷也被称为硫醇探针，是一种通过光解反应激活的荧光试剂。溴乙烷在溶液中与小硫醇基团（比如 GSH）和有反应活性的蛋白硫醇基团（如 血红蛋白）反应。 溴乙烷与硫醇的反应是二级反应，和pH相关。并且，通过与硫醇反应，激活了用于检测的水溶性荧光物质。
Application
溴乙烷被用于通过HPLC测定硫醇。 它适用于作为荧光测定2,3-二巯基丙烷-1-磺酸和其他二硫醇的柱前衍生试剂。 溴乙烷被用做研究牛心脏线粒体中对寡霉素敏感的ATP酶的荧光标记。
Features and Benefits
用溴乙烷分析低分子量硫醇的好处在于： 高选择性。
- 反应快速。
- 易于通过反相HPLC分离衍生物。
- 能穿透细胞。
Packaging
无底玻璃瓶。内含物在插入的融合锥体内。
Other Notes
荧光硫醇特异性试剂
存储类别
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
P C Chinn et al.
Analytical biochemistry, 159(1), 143-149 (1986-11-15)
A highly sensitive and specific assay for Escherichia coli thioredoxin was developed using the thiol-specific reagent monobromobimane. Treatment of dithiothreitol-reduced thioredoxin with an excess of monobromobimane in Tris buffer (pH 8.0, 23 degrees C) for 30 min resulted in the
R M Maiorino et al.
Journal of chromatography, 374(2), 297-310 (1986-01-24)
The increasing therapeutic use of dithiol metal binding agents, such as 2,3-dimercaptopropane-1-sulfonic acid (DMPS), has stimulated the need for a sensitive and selective method for their determination in biological fluids. A method has now been developed in which DMPS was
Determination of low-molecular-weight thiols using monobromobimane fluorescent labeling and high-performance liquid chromatography.
R C Fahey et al.
Methods in enzymology, 143, 85-96 (1987-01-01)
Brigitte Bauer et al.
Chemical research in toxicology, 24(5), 737-743 (2011-04-14)
Allergic contact dermatitis is the most prevalent form of human immunotoxicity. It is caused by reactive low molecular weight chemicals, that is, haptens, coming in contact with the skin where hapten-peptide complexes are formed, activating the immune system. By using
Michael Hall et al.
Proteomics, 10(5), 987-1001 (2010-01-06)
The light-dependent regulation of stromal enzymes by thioredoxin (Trx)-catalysed disulphide/dithiol exchange is known as a classical mechanism for control of chloroplast metabolism. Recent proteome studies show that Trx targets are present not only in the stroma but in all chloroplast
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