72755
5-(Bromomethyl)fluorescein
BioReagent, suitable for fluorescence, ≥95% (sum of tautomers, GC)
别名:
5-BMF
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关于此项目
经验公式（希尔记法）:
C21H13BrO5
化学文摘社编号:
分子量:
425.23
PubChem Substance ID:
UNSPSC Code:
12352200
InChI
1S/C21H13BrO5/c22-10-11-1-4-15-14(7-11)20(25)27-21(15)16-5-2-12(23)8-18(16)26-19-9-13(24)3-6-17(19)21/h1-9,23-24H,10H2
InChI key
OQHKPJAZGYJYTB-UHFFFAOYSA-N
SMILES string
Oc1ccc2c(Oc3cc(O)ccc3C24OC(=O)c5cc(CBr)ccc45)c1
product line
BioReagent
assay
≥95% (sum of tautomers, GC)
fluorescence
λex 492 nm; λem 515 nm in 0.1 M Tris pH 9.0
suitability
suitable for fluorescence
Packaging
Bottomless glass bottle. Contents are inside inserted fused cone.
Other Notes
Labeling of nucleosides and nucleotides at the N-atom; labeling of carboxylic acids: shows very strong absorption and fluorescence.
存储类别
13 - Non Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
法规信息
新产品
此项目有
P S Mukherjee et al.
Pharmaceutical research, 12(6), 930-936 (1995-06-01)
5-Bromomethyl fluorescein (5-BMF) was evaluated in this work as a pre-column, off-line derivatizing reagent for analytes containing a free carboxyl group. The reagent possessed high molar absorptivity and quantum yield and it's excitation maximum matched the intense 488.0 nm emission
U Alexiev et al.
Proceedings of the National Academy of Sciences of the United States of America, 92(2), 372-376 (1995-01-17)
The pH-indicator dye fluorescein was covalently bound to the surface of the purple membrane at position 72 on the extracellular side of bacteriorhopsin and at positions 101, 105, 160, or 231 on the cytoplasmic side by reacting bromomethylfluorescein with the
Olga Krystofova et al.
International journal of environmental research and public health, 10(1), 47-71 (2013-01-25)
Nanomaterials are structures whose exceptionality is based on their large surface, which is closely connected with reactivity and modification possibilities. Due to these properties nanomaterials are used in textile industry (antibacterial textiles with silver nanoparticles), electronics (high-resolution imaging, logical circuits
H I Stefanova et al.
Biochemistry, 32(23), 6095-6103 (1993-06-15)
The (Ca(2+)-Mg2+)-ATPase of skeletal muscle sarcoplasmic reticulum was labeled with 5-(bromomethyl)fluorescein. A stoichiometry of one label per ATPase molecule was found, which was unaffected by the presence of ATP. Labeling resulted in a 60% decrease in ATPase activity. Sequencing identified
K J Baker et al.
Biochimica et biophysica acta, 1192(1), 53-60 (1994-06-01)
The Ca(2+)-ATPase of skeletal muscle sarcoplasmic reticulum can be labelled at Cys-670 and Cys-674 with 5-[[2-[(iodoacetyl) amino]ethyl]amino]naphthalene-1-sulphonic acid (IAEDANS). Resonance energy transfer has been used to measure the distance between Cys-670/Cys-674 and Glu-439 labelled with 5-(bromomethyl)fluorescein as 40 A. The
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