product line
BioReagent
manufacturer/tradename
ATTO-TEC GmbH
fluorescence
λex 647 nm; λem 680 nm in 0.1 M phosphate buffer, pH 7.0 (recommended)
suitability
suitable for fluorescence
storage temp.
2-8°C
General description
This kit contains sufficient amounts of reactive dye, buffers, and protein purification sets for performing five labeling reactions (1 mg protein each) and for the subsequent purification of the labeled protein.
Legal Information
This product is for Research use only. In case of intended commercialization, please contact the IP-holder (ATTO-TEC GmbH, Germany) for licensing.
法规信息
新产品
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Breaking the Diffraction Barrier in Fluorescence Microscopy by Optical Shelving.
Bretschneider, S.; Eggeling, Chr.; Hell, S. W.
Physical Review Letters, 98(21) (2007)
Simple buffers for 3D STORM microscopy.
van de Linde, S.; Kasper, R.; Heilemann, M.; Sauer, M.
Applied Physics B: Lasers and Optics, 93(4), 725-731 (2008)
Triple-Color Super-Resolution Imaging of Live Cells. Resolving Submicroscopic Receptor Organization in the Plasma Membrane.
Wilmes, S.; Staufenbiel, M.; Lisse, D.; Richter, Ch. P.; Beutel, O.; Busch, K. B. et al.
Angewandte Chemie (International Edition in English), 124(20), 4952-4955 (2012)
Nicolas Olivier et al.
Biomedical optics express, 4(6), 885-899 (2013-06-14)
3D STORM is one of the leading methods for super-resolution imaging, with resolution down to 10 nm in the lateral direction, and 30-50 nm in the axial direction. However, there is one important requirement to perform this type of imaging:
Direct stochastic optical reconstruction microscopy with standard fluorescent probes.
van de Linde, S.; et al.
Nature Protocols, 6(7), 991-1009 (2011)
商品
Protein labeling kits with Atto and Tracy dyes provide easy fluorescent labeling of purified proteins, enzymes, and antibodies.
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