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Merck
CN

75117

Laccase from Pleurotus ostreatus (mushroom)

≥4.0 U/mg

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化学文摘社编号:
UNSPSC Code:
12352204
EC Number:
420-150-4
MDL number:
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form

crystals, powder

specific activity

≥4.0 U/mg

storage temp.

−20°C

Application

Laccase is polyphenol oxidase found in many plants, fungi and microorganisms. Laccases may be useful in enzymatic biofuel systems, teeth whitening, textile dyeing, and in other applications that require the removal of oxygen .

Biochem/physiol Actions

Laccase is a blue copper oxidase that reduces molecular oxygen to water. Laccase oxidizes polyphenols, methoxy-substituted phenols and diamines, but not tyrosine. Oxidation by laccase is an one-electron reaction that generates a free radical . Laccase contains 4 copper atoms/molecule which are distributed in three different copper binding sites .

Packaging

Bottomless glass bottle. Contents are inside inserted fused cone.

Other Notes

1 U corresponds to the amount of enzyme which converts 1 μmol of pyrocatechol per minute at pH 4.5 and 25°C

pictograms

Health hazard

signalword

Danger

hcodes

Hazard Classifications

Resp. Sens. 1

存储类别

13 - Non Combustible Solids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

法规信息

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分析证书(COA)

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G Palmieri et al.
The Journal of biological chemistry, 272(50), 31301-31307 (1998-02-12)
Two laccase isoenzymes (POXA1 and POXA2) produced by Pleurotus ostreatus were purified and fully characterized. POXA1 and POXA2 are monomeric glycoproteins with 3 and 9% carbohydrate content, molecular masses of about 61 and 67 kDa by sodium dodecyl sulfate polyacrylamide
The structure and function of fungal laccases
Christopher F. Thurston
Microbiology, 140, 19-26 (1994)
Mohammed Sherif et al.
Microbial biotechnology, 6(5), 588-597 (2013-07-03)
Multicopper oxidases can act on a broad spectrum of phenolic and non-phenolic compounds. These enzymes include laccases, which are widely distributed in plants and fungi, and were more recently identified in bacteria. Here, we present the results of biochemical and
Yu-Chan Zhang et al.
Nature biotechnology, 31(9), 848-852 (2013-07-23)
Increasing grain yields is a major focus of crop breeders around the world. Here we report that overexpression of the rice microRNA (miRNA) OsmiR397, which is naturally highly expressed in young panicles and grains, enlarges grain size and promotes panicle
Ngoc Han Tran et al.
Bioresource technology, 146, 721-731 (2013-08-21)
Many efforts have been made to understand the biodegradation of emerging trace organic contaminants (EOCs) in the natural and engineered systems. This review summarizes the current knowledge on the biodegradation of EOCs while having in-depth discussion on metabolism and cometabolism

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