75164
4-甲基伞形酮油酸酯
lipase substrate, ≥95% (HPCE), solid, suitable for fluorescence
别名:
油酸 4-甲基伞形酮酯
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关于此项目
经验公式(希尔记法):
C28H40O4
CAS Number:
分子量:
440.61
Beilstein:
4887028
EC 号:
MDL编号:
UNSPSC代码:
12352204
PubChem化学物质编号:
NACRES:
NA.21
Product Name
4-甲基伞形酮油酸酯, suitable for fluorescence, ≥95% (HPCE)
质量水平
方案
≥95% (HPCE)
表单
solid
储存条件
protect from light
under inert gas
mp
37-38 °C (lit.)
溶解性
2-methoxyethanol: soluble
DMSO: soluble
chloroform: soluble
荧光
λex 312 nm in methanol
λex 327 nm; λem 449 nm in 0.1 M phosphate pH 7.0 (lipase)
适用性
suitable for fluorescence
运输
wet ice
储存温度
−20°C
SMILES字符串
CCCCCCCC\C=C/CCCCCCCC(=O)Oc1ccc2C(C)=CC(=O)Oc2c1
InChI
1S/C28H40O4/c1-3-4-5-6-7-8-9-10-11-12-13-14-15-16-17-18-27(29)31-24-19-20-25-23(2)21-28(30)32-26(25)22-24/h10-11,19-22H,3-9,12-18H2,1-2H3/b11-10-
InChI key
NKQFKJYKCVDLPT-KHPPLWFESA-N
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应用
4-甲基伞形油基油酸酯已被用于:
- 作为测定非特异性酯酶活性的底物
- 确定脂肪酶活性
- 作为评估胰腺脂肪酶抑制活性的底物
包装
无底玻璃瓶。内含物在插入的融合锥体内。
其他说明
脂肪酶的荧光底物
储存分类代码
11 - Combustible Solids
WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
个人防护装备
Eyeshields, Gloves, type N95 (US)
T Imanaka et al.
Biochimica et biophysica acta, 665(2), 322-330 (1981-08-24)
An acid lipase was purified from rabbit liver lysosomes by, in sequence, osmotic treatment of the lysosomal fraction, Sephadex LH-20, DEAE-Sephadex A-50, Bio-Gel A-5m, hydroxyapatite and, finally, Sephadex G-200 column chromatography. The substrate was 4-methylumbelliferyl oleate. The enzyme was solubilized
Ola Abdelhedi et al.
Marine drugs, 17(4) (2019-04-03)
In this study, the antihypertensive activity of Purafect®-smooth hound viscera protein hydrolysate (VPH) and its peptide fraction with molecular weight (MW) below 1 kDa (VPH-I) was investigated. In addition, the lipase inhibitory activity, as well the anticoagulant potential, in vitro
Study of the hydrolysis of 4-methylumbelliferyl oleate by acid lipase and cholesteryl oleate by acid cholesteryl esterase in human leucocytes, fibroblasts and liver.
J F Koster et al.
Biochimica et biophysica acta, 618(1), 98-105 (1980-04-18)
Thavaree Thilavech et al.
BMC complementary and alternative medicine, 19(1), 242-242 (2019-09-07)
Cyanidin-3-rutinoside (C3R), a naturally occurring anthocyanin, possesses anti-oxidant, anti-hyperglycemic, anti-glycation and cardioprotective properties. However, its mechanisms responsible for anti-hyperlipidemic activity have not been fully identified. The aim of the study was to investigate the lipid-lowering mechanisms of C3R through inhibition
T J Van Berkel et al.
Biochimica et biophysica acta, 617(3), 446-457 (1980-03-21)
(1) Parenchymal and non-parenchymal cells were isolated from rat liver. The characteristics of acid lipase activity with 4-methylumbelliferyl oleate as substrate and acid cholesteryl esterase activity with cholesteryl[1-14C]oleate as substrate were investigated. The substrates were incorporated in egg yolk lecithin
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