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Merck
CN

75164

Sigma-Aldrich

4-甲基伞形酮油酸酯

lipase substrate, ≥95% (HPCE), solid, suitable for fluorescence

别名:

油酸 4-甲基伞形酮酯

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关于此项目

经验公式(希尔记法):
C28H40O4
化学文摘社编号:
分子量:
440.61
Beilstein:
4887028
EC 号:
MDL编号:
UNSPSC代码:
12352204
PubChem化学物质编号:
NACRES:
NA.21
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产品名称

4-甲基伞形酮油酸酯, suitable for fluorescence, ≥95% (HPCE)

质量水平

方案

≥95% (HPCE)

表单

solid

储存条件

protect from light
under inert gas

mp

37-38 °C (lit.)

溶解性

2-methoxyethanol: soluble
DMSO: soluble
chloroform: soluble

荧光

λex 312 nm in methanol
λex 327 nm; λem 449 nm in 0.1 M phosphate pH 7.0 (lipase)

适用性

suitable for fluorescence

运输

wet ice

储存温度

−20°C

SMILES字符串

CCCCCCCC\C=C/CCCCCCCC(=O)Oc1ccc2C(C)=CC(=O)Oc2c1

InChI

1S/C28H40O4/c1-3-4-5-6-7-8-9-10-11-12-13-14-15-16-17-18-27(29)31-24-19-20-25-23(2)21-28(30)32-26(25)22-24/h10-11,19-22H,3-9,12-18H2,1-2H3/b11-10-

InChI key

NKQFKJYKCVDLPT-KHPPLWFESA-N

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应用

4-甲基伞形油基油酸酯已被用于:
  • 作为测定非特异性酯酶活性的底物
  • 确定脂肪酶活性
  • 作为评估胰腺脂肪酶抑制活性的底物

包装

无底玻璃瓶。内含物在插入的融合锥体内。

其他说明

脂肪酶的荧光底物

储存分类代码

11 - Combustible Solids

WGK

WGK 3

闪点(°F)

Not applicable

闪点(°C)

Not applicable

个人防护装备

Eyeshields, Gloves, type N95 (US)


历史批次信息供参考:

分析证书(COA)

Lot/Batch Number

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T J Van Berkel et al.
Biochimica et biophysica acta, 617(3), 446-457 (1980-03-21)
(1) Parenchymal and non-parenchymal cells were isolated from rat liver. The characteristics of acid lipase activity with 4-methylumbelliferyl oleate as substrate and acid cholesteryl esterase activity with cholesteryl[1-14C]oleate as substrate were investigated. The substrates were incorporated in egg yolk lecithin
Non-flavonoid phenolics from Averrhoa carambola fresh fruit
Jia X, et al.
Journal of functional foods, 32(1), 419-425 (2017)
Fluorometric assay for the hydrolytic activity of lipase using fatty acyl esters of 4-methylumbelliferone.
T J Jacks et al.
Analytical biochemistry, 21(2), 279-285 (1967-11-01)
Lysosomal acid lipase in cultivated fibroblasts: characterization of enzyme activity in normal and enzymatically deficient cell lines.
B K Burton et al.
Clinica chimica acta; international journal of clinical chemistry, 101(1), 25-32 (1980-02-14)
Tao Chen et al.
Meat science, 84(1), 143-146 (2010-04-09)
In order to reveal the relationship between phospholipase A(2) and antioxidant enzymes and drip loss in pork, the study was designed to examine the effects of phospholipase A(2) and antioxidant enzymes on the water-holding capacity of pork during postmortem chilling.

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