75926
Oxonol VI
suitable for fluorescence, ≥95% (UV)
别名:
1,5-Bis(5-oxo-3-propylisoxazol-4-yl)pentamethine oxonol
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关于此项目
经验公式（希尔记法）:
C17H20N2O4
化学文摘社编号:
分子量:
316.35
NACRES:
NA.32
PubChem Substance ID:
UNSPSC Code:
12171500
MDL number:
InChI
1S/C17H20N2O4/c1-3-8-14-12(16(20)22-18-14)10-6-5-7-11-13-15(9-4-2)19-23-17(13)21/h5-7,10-11,20H,3-4,8-9H2,1-2H3/b7-5+,10-6+,13-11-
SMILES string
CCCC1=NOC(=O)\C1=C/C=C/C=C/c2c(O)onc2CCC
InChI key
CPHARWHMAGUXDW-PQWGHOHWSA-N
assay
≥95% (UV)
form
powder
solubility
methanol: soluble
fluorescence
λex 523 nm; λem 630 nm in 0.1 M Tris pH 9.0
suitability
suitable for fluorescence
shipped in
wet ice
storage temp.
−20°C
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Application
Oxonol VI can be used as a fluorescent potentiometric indicator to detect the membrane potentials in lipid vesicles. Quantitative measurements of the membrane potentials can be easily calibrated using oxonol VI because of its response to membrane potential changes. It is also used as an electric potential-sensitive probe to study membrane vesicles from E. coli cells.
Packaging
Bottomless glass bottle. Contents are inside inserted fused cone.
Other Notes
As an optical indicator for membrane potentials in lipid vesicles
存储类别
13 - Non Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
Ratiometric fluorescence measurements of membrane potential generated by yeast plasma membrane H+-ATPase reconstituted into vesicles
Holoubek A, et al.
Biochimica et Biophysica Acta - Biomembranes, 1609(1), 71-79 (2003)
Selective electrodiffusion of zinc ions in a Zrt-, Irt-like protein, ZIPB
Lin W, et al.
Journal of biological and chemical chronicles, 285(50), 39013-39020 (2010)
Preparation of Everted Membrane Vesicles from Escherichia coli Cells
Verkhovskaya M
Bio-protocol, 7(9), e2254-e2254 (2017)
J Fumero et al.
Biochimica et biophysica acta, 944(2), 164-176 (1988-10-06)
Differential scanning calorimetry (DSC) has been employed to determine the effect of five commonly employed extrinsic potential-sensitive probes on phase transitions of multilamellar suspensions of L-alpha-dimyristoylphosphatidylcholine (DMPC). At mol% values of less than five, the effect of these probes on
R J Clarke et al.
Biochimica et biophysica acta, 981(2), 326-336 (1989-06-06)
A method is described for studying the coupling ratio of the Na+/K+ pump, i.e., the ratio of pump-mediated fluxes of Na+ and K+, in a reconstituted system. The method is based on the comparison of the pump-generated current with the
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