77152
胃蛋白酶 来源于猪胃粘膜
2× cryst., lyophilized, powder, ≥2400 U/mg
别名:
胃液素 来源于猪胃, 胃蛋白酶 A
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应用
胃蛋白酶切割可用于产生抗体的F(ab′)2 片段。有关胃蛋白酶的更多信息，请访问 www.sigma-aldrich.com/enzymeexplorer。
生化/生理作用
优先裂解：P1 和 P1′ 位的疏水性和芳族残基。降解胰岛素 β 链中的 Phe-Val、Gln-His、Glu-Ala、Ala-Leu、Leu-Tyr、Tyr-Leu、Gly-Phe、Phe-Phe 和 Phe-Tyr 键
分析说明
最适pH值为2-4。在4 M尿素和3 M盐酸胍中可保持活性。在60°C时稳定。胃蛋白酶在pH 8.0-8.5下发生不可逆灭活。
其他说明
1 U corresponds to the amount of enzyme which increases the absorbance at 280 nm by 0.001 per minute at pH 2.0 and 37°C (Hemoglobin, Cat. No. 51290, as substrate) 15′000 absorbance-U as described above are equivalent to ∼1 Bergmeyer-U. 1 Bergmeyer-U is the amount of enzyme which hydrolyzes 1 μmol Acetyl-L-phenyl-3,5-diiodo-L-tyrosine at pH 2.0 and 37°C
Sales restrictions may apply
Standard for serum pepsinogen determination; Substrate specificity; Limited hydrolysis of myelin basic protein
警示用语：
Danger
危险分类
Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
靶器官
Respiratory system
储存分类代码
11 - Combustible Solids
WGK
WGK 1
闪点(°F)
Not applicable
闪点(°C)
Not applicable
个人防护装备
dust mask type N95 (US), Eyeshields, Faceshields, Gloves
法规信息
新产品
此项目有
J D Harvey-White et al.
American journal of veterinary research, 43(7), 1317-1320 (1982-07-01)
A simplification of the traditional hemoglobin methods for determining serum pepsinogen concentration was developed. In this method, 10% trichloroacetic acid solution was added to control samples, and hemoglobin substrate was added to controls and active enzyme samples; standards and samples
R E Martenson et al.
Journal of neurochemistry, 37(6), 1497-1508 (1981-12-01)
Treatment of rabbit myelin basic protein component 1 with pepsin (enzyme:substrate, 1:500 w/w) in 0.5 M-ammonium formate (pH 6.00) for 15-20 min at room temperature resulted in limited cleavage of the protein. The resulting fragments were isolated by ion-exchange chromatography
K. Morihara et al.
Proteinases and their inhibitors, V. Turk et al., eds.,, 213-213 (1981)
Chuan-Hsiao Han et al.
Food chemistry, 138(2-3), 923-930 (2013-02-16)
Our previous report showed that yam dioscorin and its peptic hydrolysates exhibit radical scavenging activities; however, the functions of these peptic hydrolases are still under investigation. In this study, the thiol-containing peptides derived from computer-aided simulation of pepsin hydrolysis of
Martial Rey et al.
Molecular & cellular proteomics : MCP, 12(2), 464-472 (2012-12-01)
Studies of protein dynamics, structure and interactions using hydrogen/deuterium exchange mass spectrometry (HDX-MS) have sharply increased over the past 5-10 years. The predominant technology requires fast digestion at pH 2-3 to retain deuterium label. Pepsin is used almost exclusively, but
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