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Merck
CN

77398

(R)-Oxynitrilase, Linum usitatissimum, recombinant from Pichia pastoris

solution (clear), slightly yellow, ≥50 U/mL

别名:

(R)-Acetone cyanohydrin Lyase

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UNSPSC Code:
12352204
MDL number:
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recombinant

expressed in Pichia pastoris

form

solution (clear)

concentration

≥50 U/mL

color

slightly yellow

storage temp.

2-8°C

Biochem/physiol Actions

R-oxynitrilase catalyzes the enantioselective addition of HCN to aldehydes or ketones.

Other Notes

1 U corresponds to the amount of enzyme which cleaves 1 μmol acetone cyanhydrin to cyanide and acetone per minute at pH 4.2 and 30 °C
Investigation of the substrate range
contains ~20 mM sodium acetate, ~20 mM ZnCl2, ~1 M NaCl, pH ~5.7

pictograms

Health hazard

signalword

Danger

hcodes

Hazard Classifications

Resp. Sens. 1 - Skin Sens. 1

存储类别

12 - Non Combustible Liquids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Faceshields, Gloves

法规信息

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J Albrecht et al.
Biotechnology and applied biochemistry, 17 ( Pt 2), 191-203 (1993-04-01)
The purification of (R)-oxynitrilase (EC 4.1.2.10) from Linum usitatissimum (flax) has been improved considerably. The enzyme is obtained from seedlings in 60% yield by fractional salt precipitation followed by ion-exchange and hydrophobic-interaction chromatography. Final gel-permeation chromatography yields a protein with

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