79385
磷酸酶(碱性) 来源于牛肠粘膜
solution (clear), >10000 U/mL
别名:
Alkaline Phosphatase from calf intestinal mucosa
表单
solution (clear)
比活
≥1000 U/mg protein
分子量
Mr ~140000
浓度
>10000 U/mL
密度
1.12 g/mL at 20 °C
储存温度
2-8°C
应用
Alkaline phosphatase is used in the laboratory to remove phosphate monoesters which prevents self-ligation. It is also used in the dairy industry to confirm pasturization of milk. Alkaline phosphatse is commonly conjugated to antibodies and used in immunoblotting, ELISA′s, and other antibody based detection assays.
生化/生理作用
Alkaline phosphatase is a hydrolytic enzyme which dephosphorylates a variety of molecules under basic conditions.
外形
solution in 3 M NaCl, 1 mM MgCl2, 0.1 mM ZnCl2, 30 mM triethanolamine, pH 7.6
其他说明
1 U corresponds to the amount of enzyme which hydrolyzes 1 μmol 4-nitrophenyl phosphate per minute at pH 9.8 and 37°C
警示用语：
Danger
危险声明
预防措施声明
危险分类
Resp. Sens. 1
储存分类代码
10 - Combustible liquids
WGK
WGK 1
闪点(°F)
Not applicable
闪点(°C)
Not applicable
个人防护装备
Eyeshields, Gloves, type N95 (US)
法规信息
新产品
此项目有
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American journal of human genetics, 92(4), 584-589 (2013-04-09)
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Gouri Chaudhuri et al.
Indian journal of biochemistry & biophysics, 50(1), 64-71 (2013-04-27)
The hydrolysis of p-nitrophenyl phosphate (pNPP) by calf intestinal alkaline phosphatase (CIAP) was investigated with respect to kinetic parameters such as V(max), K(m) and K(cat) under varying pH, buffers, substrate concentration, temperature and period of incubation. Highest activity was obtained
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