83636
Resorufin acetate
suitable for fluorescence, ≥98.0% (TLC)
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经验公式（希尔记法）:
C14H9NO4
化学文摘社编号:
分子量:
255.23
UNSPSC Code:
12352200
NACRES:
NA.32
PubChem Substance ID:
MDL number:
Beilstein/REAXYS Number:
246139
产品名称
Resorufin acetate, suitable for fluorescence, ≥98.0% (TLC)
InChI
1S/C14H9NO4/c1-8(16)18-10-3-5-12-14(7-10)19-13-6-9(17)2-4-11(13)15-12/h2-7H,1H3
SMILES string
CC(=O)Oc1ccc2N=C3C=CC(=O)C=C3Oc2c1
InChI key
UJWKHDKBOVPINX-UHFFFAOYSA-N
assay
≥98.0% (TLC)
form
powder or crystals
mp
223 °C (lit.)
solubility
DMF: soluble
DMSO: soluble
alcohols: soluble
fluorescence
λex 500 nm; λem 593 nm in 0.1 M phosphate pH 8.0 (chymotrypsin)
λex 540 nm; λem 590 nm in DMSO
λex 571 nm; λem 584 nm (Reaction product)
suitability
suitable for fluorescence
storage temp.
2-8°C
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Application
suitable as fluorogenic substrate for hydrolytic enzymes (cellulases, chymotrypsin)
Other Notes
Studies on the esterase activity of cytosolic aldehyde dehydrogenase
signalword
Warning
hcodes
Hazard Classifications
Acute Tox. 4 Oral - Eye Irrit. 2 - Skin Irrit. 2
存储类别
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
dust mask type N95 (US), Eyeshields, Gloves
法规信息
新产品
此项目有
Myung Gil Choi et al.
Organic letters, 12(7), 1468-1471 (2010-03-13)
The acetate derivatives of fluorescein and resorufin exhibited a prominent turn-on type signaling behavior toward BO(3)(-) ions over other common anions. Signaling is based on the selective deprotection of acetate groups by perborate, which resulted in significant chromogenic and fluorogenic
The action of cytosolic aldehyde dehydrogenase on resorufin acetate.
T M Kitson et al.
Advances in experimental medicine and biology, 414, 201-208 (1997-01-01)
T M Kitson
Biochimica et biophysica acta, 1385(1), 43-52 (1998-06-19)
Resorufin bromoacetate is a substrate that is rapidly hydrolysed by chymotrypsin. The reaction shows a pre-steady-state burst phase that may be observed by stopped flow spectrophotometry if precautions are taken against spontaneous hydrolysis of the substrate. The strongly activating effect
T M Kitson et al.
The Biochemical journal, 322 ( Pt 3), 701-708 (1997-03-15)
Resorufin acetate is a very good substrate for sheep liver cytosolic aldehyde dehydrogenase, both from the point of view of practical spectrophotometry and in terms of information provided about the nature of the catalysis shown by this enzyme. p-Nitrophenyl (PNP)
Mohit P Mathew et al.
Chembiochem : a European journal of chemical biology, 18(13), 1204-1215 (2017-02-22)
This report describes the metabolic glycoengineering (MGE) of intracellular esterase activity in human colon cancer (LS174T) and Chinese hamster ovary (CHO) cells. In silico analysis of carboxylesterases CES1 and CES2 suggested that these enzymes are modified with sialylated N-glycans, which
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