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Merck
CN

89827

Fluorescent IEF-Marker pI 4.0 solution

1 mg/mL in H2O

别名:

IEF-Marker fluorescent pI 4.0

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MDL number:
UNSPSC Code:
12352200
NACRES:
NA.26
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mol wt

Mw 250.29 g/mol

concentration

1 mg/mL in H2O

fluorescence

λex 310 nm; λem 415 nm in 0.1 M citrate pH 4.0

application(s)

cleaning products
cosmetics
flavors and fragrances
food and beverages
personal care

storage temp.

2-8°C

General description

filtered through a 0.45 μm membrane filter

Application

Suitable as IEF-Marker for HPCE and for IEF-gels

Preparation Note

For use in HPCE the marker should be diluted 1:100 in a suitable ampholyte solution (e.g. 2% ampholyte 3-10), UV-detection at 280 nm (20 °C). For use in IEF-gels the solution can be loaded directly onto the gel (max. 1μl per lane).


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存储类别

12 - Non Combustible Liquids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

法规信息

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商品

Information on Isoelectric Focusing including what it is and how it is used. In order to ensure the high performance of analysis, isoelectric point (pI) standards are needed.


Minh Khang Chau et al.
Analytica chimica acta, 1124, 60-70 (2020-06-15)
We report capacitively coupled contactless conductivity detection (C4D) of proteins separated by microfluidic capillary isoelectric focusing (μCIEF). To elucidate the evolution of negative conductivity peaks during focusing and seek IEF conditions for sensitive conductivity detection, numerical simulation was performed using



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