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Merck
CN

90308

Sigma-Aldrich

deGlycIT MicroSpin

deglycosylates up to 0.5 mg IgG, 5 columns

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UNSPSC代码:
12352204
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表单

suspension

储存温度

2-8°C

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一般描述

5 x 0.1 ml settled resin of endoglycosidase IgGZERO (EndoS) immobilized on highly crosslinked agarose. Agarose resin is suspended in 20% ethanol/water (V/V)

应用

DeGlycIT MicroSpin columns (deglycosylates up to 0.5 mg IgG, 5 columns) can be used for antibody characterization.

生化/生理作用

DeGlycIT MicroSpin columns comprise of pre-filled endoglycosidase IgGZERO (EndoS) enzyme covalently coupled to highly cross-linked agarose. IgGZERO is a bacterial enzyme isolated from Streptococcus pyogenes, which possess hydrolytic activity specific for IgG bound glycans and cleaves bound IgG from IgG Fc receptors without damaging the native cells.

分析说明

Enzymatic activity: corresponds to requirements

其他说明

1 ml of settled enzyme immobilized agarose resin removes 95% of 5 mg of human IgG Fc glycans in 15 minutes at 25°C, pH 7.2 as monitored by SDS-PAGE.

象形图

Flame

警示用语:

Danger

危险声明

危险分类

Flam. Liq. 2

储存分类代码

3 - Flammable liquids

WGK

WGK 3

闪点(°F)

57.2 °F - closed cup

闪点(°C)

14.0 °C - closed cup

法规信息

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分析证书(COA)

Lot/Batch Number

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Michele S Y Tan et al.
PloS one, 15(1), e0227341-e0227341 (2020-01-11)
Clan CA cysteine proteases, also known as papain-like proteases, play important roles throughout the malaria parasite life cycle and are therefore potential drug targets to treat this disease and prevent its transmission. In order to study the biological function of
Jonathan J Goodfellow et al.
Journal of the American Chemical Society, 134(19), 8030-8033 (2012-05-04)
Protein endoglycosidases are useful for biocatalytic alteration of glycans on protein surfaces, but the currently limited selectivity of endoglycosidases has prevented effective manipulation of certain N-linked glycans widely found in nature. Here we reveal that a bacterial endoglycosidase from Streptococcus

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