表单
suspension
储存温度
2-8°C
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一般描述
5 x 0.1 ml settled resin of endoglycosidase IgGZERO (EndoS) immobilized on highly crosslinked agarose. Agarose resin is suspended in 20% ethanol/water (V/V)
应用
DeGlycIT MicroSpin columns (deglycosylates up to 0.5 mg IgG, 5 columns) can be used for antibody characterization.
生化/生理作用
DeGlycIT MicroSpin columns comprise of pre-filled endoglycosidase IgGZERO (EndoS) enzyme covalently coupled to highly cross-linked agarose. IgGZERO is a bacterial enzyme isolated from Streptococcus pyogenes, which possess hydrolytic activity specific for IgG bound glycans and cleaves bound IgG from IgG Fc receptors without damaging the native cells.
分析说明
Enzymatic activity: corresponds to requirements
其他说明
1 ml of settled enzyme immobilized agarose resin removes 95% of 5 mg of human IgG Fc glycans in 15 minutes at 25°C, pH 7.2 as monitored by SDS-PAGE.
警示用语:
Danger
危险声明
危险分类
Flam. Liq. 2
储存分类代码
3 - Flammable liquids
WGK
WGK 3
闪点(°F)
57.2 °F - closed cup
闪点(°C)
14.0 °C - closed cup
法规信息
新产品
此项目有
Michele S Y Tan et al.
PloS one, 15(1), e0227341-e0227341 (2020-01-11)
Clan CA cysteine proteases, also known as papain-like proteases, play important roles throughout the malaria parasite life cycle and are therefore potential drug targets to treat this disease and prevent its transmission. In order to study the biological function of
Jonathan J Goodfellow et al.
Journal of the American Chemical Society, 134(19), 8030-8033 (2012-05-04)
Protein endoglycosidases are useful for biocatalytic alteration of glycans on protein surfaces, but the currently limited selectivity of endoglycosidases has prevented effective manipulation of certain N-linked glycans widely found in nature. Here we reveal that a bacterial endoglycosidase from Streptococcus
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