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Merck
CN

91252

Sigma-Aldrich

2-Deoxyribose 5-phosphate Aldolase, E. coli K12, recombinant K12 from E. coli

≥2 U/mg

别名:

DERA, Deoxyriboaldolase

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化学文摘社编号:
EC 号:
4.1.2.4.
MDL编号:
UNSPSC代码:
12352204
NACRES:
NA.54
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重组

expressed in E. coli

质量水平

表单

crystals
powder

比活

≥2 U/mg

运输

wet ice

储存温度

−20°C

生化/生理作用

Deoxyriboaldolase catalyzes the condensation of acetaldehyde and glyceraldehyde 3-phosphate to 2-deoxyribose 5-phosphate.

其他说明

1 U corresponds to the amount of enzyme which converts 1 μmol 2-Deoxy-D-ribose-5-phosphate per minute at pH 7.5 and 25°C
The enzyme catalyzes the reversible cleavage of 2-deoxyribose-5-phosphate to acetaldehyde and D-glyceraldehyde-3-phosphate

储存分类代码

13 - Non Combustible Solids

WGK

WGK 3

闪点(°F)

Not applicable

闪点(°C)

Not applicable

个人防护装备

Eyeshields, Gloves, type N95 (US)

法规信息

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历史批次信息供参考:

分析证书(COA)

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Robert A Alberty
Biochemistry, 43(30), 9840-9845 (2004-07-28)
Recent thermodynamic measurements have made it possible to calculate the apparent equilibrium constants of the ribonucleoside diphosphate reductase reaction and the ribonucleoside triphosphate reductase reaction with various reducing agents. Third law heat capacity measurements on crystals of d-ribose and other
Francisco S Domingues et al.
Protein engineering, design & selection : PEDS, 17(6), 537-543 (2004-08-21)
Experimentally determined protein structures have been classified in different public databases according to their structural and evolutionary relationships. Frequently, alternative structural models, determined using X-ray crystallography or NMR spectroscopy, are available for a protein. These models can present significant structural
Yong-Mo Kim et al.
Journal of microbiology and biotechnology, 20(6), 995-1000 (2010-07-14)
A novel deoC gene was identified from Paenibacillus sp. EA001 isolated from soil. The gene had an open reading frame (ORF) of 663 base pairs encoding 220 amino acids with a molecular mass of 24.5 kDa. The amino acid sequence
A Heine et al.
Science (New York, N.Y.), 294(5541), 369-374 (2001-10-13)
In classical enzymology, intermediates and transition states in a catalytic mechanism are usually inferred from a series of biochemical experiments. Here, we derive an enzyme mechanism from true atomic-resolution x-ray structures of reaction intermediates. Two ultra-high resolution structures of wild-type
Xiaolin Pei et al.
Applied biochemistry and biotechnology, 162(5), 1423-1434 (2010-03-17)
2-Deoxyribose-5-phosphate aldolase (DERA) catalyzes a sequential aldol reaction useful in synthetic chemistry. In this work, the effect of a feeding strategy on the production of a thermophilic DERA was investigated in fed-batch cultures of recombinant Escherichia coli BL21 (pET303-DERA008). The

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