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Merck
CN

91252

2-Deoxyribose 5-phosphate Aldolase, E. coli K12, recombinant K12 from E. coli

≥2 U/mg

别名:

DERA, Deoxyriboaldolase

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关于此项目

化学文摘社编号:
UNSPSC Code:
12352204
NACRES:
NA.54
EC 号:
4.1.2.4.
MDL number:
Specific activity:
≥2 U/mg
Recombinant:
expressed in E. coli
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recombinant

expressed in E. coli

Quality Level

form

crystals, powder

specific activity

≥2 U/mg

shipped in

wet ice

storage temp.

−20°C

Biochem/physiol Actions

Deoxyriboaldolase catalyzes the condensation of acetaldehyde and glyceraldehyde 3-phosphate to 2-deoxyribose 5-phosphate.

Other Notes

1 U corresponds to the amount of enzyme which converts 1 μmol 2-Deoxy-D-ribose-5-phosphate per minute at pH 7.5 and 25°C
The enzyme catalyzes the reversible cleavage of 2-deoxyribose-5-phosphate to acetaldehyde and D-glyceraldehyde-3-phosphate


存储类别

13 - Non Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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Robert A Alberty
Biochemistry, 43(30), 9840-9845 (2004-07-28)
Recent thermodynamic measurements have made it possible to calculate the apparent equilibrium constants of the ribonucleoside diphosphate reductase reaction and the ribonucleoside triphosphate reductase reaction with various reducing agents. Third law heat capacity measurements on crystals of d-ribose and other
Francisco S Domingues et al.
Protein engineering, design & selection : PEDS, 17(6), 537-543 (2004-08-21)
Experimentally determined protein structures have been classified in different public databases according to their structural and evolutionary relationships. Frequently, alternative structural models, determined using X-ray crystallography or NMR spectroscopy, are available for a protein. These models can present significant structural
Xiaolin Pei et al.
Applied biochemistry and biotechnology, 162(5), 1423-1434 (2010-03-17)
2-Deoxyribose-5-phosphate aldolase (DERA) catalyzes a sequential aldol reaction useful in synthetic chemistry. In this work, the effect of a feeding strategy on the production of a thermophilic DERA was investigated in fed-batch cultures of recombinant Escherichia coli BL21 (pET303-DERA008). The