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Merck
CN

93356

三(羟甲基)甲基甘氨酸

BioUltra, ≥99.5% (NT)

别名:

N-三(羟甲基)甲基甘氨酸

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关于此项目

线性分子式:
(HOCH2)3CNHCH2CO2H
化学文摘社编号:
分子量:
179.17
EC Number:
227-193-6
UNSPSC Code:
12161700
PubChem Substance ID:
Beilstein/REAXYS Number:
1937804
MDL number:
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product line

BioUltra

assay

≥99.5% (NT)

impurities

Insoluble matter, passes filter test

ign. residue

≤0.05% (as SO4)

loss

≤0.5% loss on drying, 110 °C

pH

4.5-6.0 (25 °C, 0.5 M in H2O)

useful pH range

7.4-8.8

pKa (25 °C)

8.1

solubility

H2O: 0.5 M at 20 °C, clear, colorless

anion traces

chloride (Cl-): ≤500 mg/kg, sulfate (SO42-): ≤50 mg/kg

cation traces

Al: ≤5 mg/kg, As: ≤0.1 mg/kg, Ba: ≤5 mg/kg, Bi: ≤5 mg/kg, Ca: ≤10 mg/kg, Cd: ≤5 mg/kg, Co: ≤5 mg/kg, Cr: ≤5 mg/kg, Cu: ≤5 mg/kg, Fe: ≤5 mg/kg, K: ≤50 mg/kg, Li: ≤5 mg/kg, Mg: ≤5 mg/kg, Mn: ≤5 mg/kg, Mo: ≤5 mg/kg, Na: ≤50 mg/kg, Ni: ≤5 mg/kg, Pb: ≤5 mg/kg, Sr: ≤5 mg/kg, Zn: ≤5 mg/kg

λ

0.5 M in H2O

UV absorption

λ: 260 nm Amax: 0.03, λ: 280 nm Amax: 0.02

SMILES string

OCC(CO)(CO)NCC(O)=O

InChI

1S/C6H13NO5/c8-2-6(3-9,4-10)7-1-5(11)12/h7-10H,1-4H2,(H,11,12)

InChI key

SEQKRHFRPICQDD-UHFFFAOYSA-N

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Application

用于分离低分子量的肽的缓冲剂组分。

Biochem/physiol Actions

Tricine, a buffer with a useful range from 7.4 to 8.8, is commonly used for the electrophoretic separation of low molecular weight proteins in gels and membranes. It is an effective buffer for ATP assays using firefly luciferase. Tricine is also an effective hydroxyl radical scavenger used to protect molecules during radical generating procedures such as irradiation.

存储类别

13 - Non Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

法规信息

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分析证书(COA)

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J.J. Webster et al.
Journal of Applied Biochemistry, 2, 469-469 (1980)
J Ambler et al.
Clinical chemistry, 26(8), 1221-1223 (1980-07-01)
Two new non-barbiturate buffers have been formulated for serum protein electrophoresis on cellulose acetate membranes. Both buffers give five distrinct fractions and are suitable for all systems, but have been primarily designed to meet the needs of the Gelman "Sepratek"
Thierry Rabilloud
Journal of proteomics, 73(8), 1562-1572 (2010-04-17)
Electrophoretic separations of proteins are widely used in proteomic analyses, and rely heavily on SDS electrophoresis. This mode of separation is almost exclusively used when a single dimension separation is performed, and generally represents the second dimension of two-dimensional separations.
Arpita Gantayet et al.
Biofouling, 29(1), 77-85 (2012-12-06)
The freshwater zebra mussel (Dreissena polymorpha) is a notorious biofouling organism. It adheres to a variety of substrata underwater by means of a proteinaceous structure called the byssus, which consists of a number of threads with adhesive plaques at the
Hermann Schägger
Nature protocols, 1(1), 16-22 (2007-04-05)
Tricine-SDS-PAGE is commonly used to separate proteins in the mass range 1-100 kDa. It is the preferred electrophoretic system for the resolution of proteins smaller than 30 kDa. The concentrations of acrylamide used in the gels are lower than in

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