A2264
β-N-乙酰氨基葡萄糖苷酶 来源于洋刀豆 （刀豆）
ammonium sulfate suspension, ≥8 units/mg protein
别名:
Β-N--乙酰- D -氨基己糖 N-乙酰氨基己糖水解酶, β-N-乙酰氨基己糖苷酶
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关于此项目
化学文摘社编号:
UNSPSC Code:
12352204
NACRES:
NA.54
EC Number:
232-730-2
MDL number:
Specific activity:
≥8 units/mg protein
Biological source:
Canavalia ensiformis
biological source
Canavalia ensiformis
form
ammonium sulfate suspension
foreign activity
α- and β-galactosidase, and α-L-fucosidase ≤0.1%, α-mannosidase ≤0.5%
storage temp.
2-8°C
Quality Level
specific activity
≥8 units/mg protein
Application
β-N-乙酰氨基葡萄糖苷酶是用于水解 N-乙酰基-β-D-氨基葡萄糖苷和 N-乙酰基-β-D-半乳糖胺的溶酶体酶。基于 β-GlcNAc 和 β-GalNAcc 的有效转糖基作用，它用于寡糖的化学酶促合成。其可能是研究阿尔茨海默病的有用工具 。来自洋刀豆的乙酰氨基葡萄糖苷酶已被用于研究生物膜的酶解离 。
Biochem/physiol Actions
这种酶，有时称为 β-N-乙酰氨基己糖苷酶，据报道可释放末端 β-连接来自多种底物的 N-乙酰葡糖胺和 N-乙酰半乳糖胺。
这种酶，有时称为 β-N-乙酰氨基己糖苷酶，据报道可释放末端 β-连接来自多种底物的 N-乙酰葡糖胺和 N-乙酰半乳糖胺。β-N-酰基葡糖胺酶的活性可以用显色底物对硝基苯基-N-乙酰基-β-D-氨基葡萄糖苷测定。 β-N-酰基葡糖胺酶水解末端非还原性 N-乙酰基-D-己糖胺残基。这种酶含有两种主要的同工酶，Hex A（异源二聚体）和 Hex B（同源二聚体）。N-乙酰氨基葡萄糖、乙酰胺、N-2-乙酰胺基-2-脱氧葡萄糖胺、N-乙酰诺吉瑞霉素和 N-乙酰脱氧诺吉瑞霉素是已知的抑制剂。
Physical form
2.5 M 混悬液 (NH4)2SO4，pH 7.0
Analysis Note
在 pH 4.0 时，对硝基苯基 β-N-乙酰氨基半乳糖在 pH 5.0 时水解速率约为对硝基苯基 β-N-乙酰氨基葡萄糖水解速率的 50%。
Other Notes
一个单位将在 pH5.0、25 °C下每分钟将 1.0μmole 对硝基苯基 N-乙酰基-β-D-氨基葡萄糖水解成对硝基苯酚和N-乙酰基-D-葡糖胺。
flash_point_f
Not applicable
flash_point_c
Not applicable
存储类别
10 - Combustible liquids
法规信息
低风险生物材料
此项目有
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商品
Instructions for working with enzymes supplied as ammonium sulfate suspensions
以硫酸铵悬浮液形式提供的酶的使用指南
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