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A3983

牛血清白蛋白来源于牛血清

Name: 牛血清白蛋白 来源于牛血清
Brand: SIGMA

lyophilized powder, essentially γ-globulin free, ≥98% (agarose gel electrophoresis)

别名:

BSA, 牛白蛋白, 牛血清白蛋白

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关于此项目

化学文摘社编号:

9048-46-8

UNSPSC Code:

12352202

EC Number:

232-936-2

NACRES:

NA.25

MDL number:

MFCD00130384

eCl@ss:

32160409

Biological source:

bovine

Assay:

≥98% (agarose gel electrophoresis)

Form:

lyophilized powder

Technique(s):

western blot: suitable

Impurities:

essentially globulin free

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属性

biological source

bovine

Quality Level

200

assay

≥98% (agarose gel electrophoresis)

form

lyophilized powder

purified by

heat shock fractionation

packaging

poly bottle of

origin

New Zealand origin

technique(s)

western blot: suitable

impurities

essentially globulin free

loss

≤5%

pH

solubility

water: soluble, clear to slightly hazy (1 g + 25 mL)

UniProt accession no.

P02769

foreign activity

Protease free

storage temp.

2-8°C

Gene Information

bovine ... ALB(280717)

说明

General description

Bovine Serum Albumin has three homologous domains (I, II, and III) that are connected by disulfide bonds. BSA shares structural homology with human serum albumin (HSA).

Application

Bovine Serum Albumin has been used:

as a component of blocking solution used for fixing Drosophila embryos in immunostaining
to block 96-deep-well plates in phage immunoprecipitation studies
as a standard solution in fluorescence spectroscopy studies

Biochem/physiol Actions

BSA的某些构象和初级序列表位是人类对牛肉和牛奶过敏中的可疑过敏原。

Bovine Serum Albumin (BSA) displays intrinsic fluorescence due to the presence of two tryptophan residues. It is widely used in ligand interaction studies and in the delivery of drugs and antibodies. BSA is used as a blocking agent in enzyme-linked immunosorbent assay (ELISA).

Preparation Note

来源于新西兰产地的牛血清

血清白蛋白可能也会被称为Fraction V。该命名惯例取自使用冷乙醇沉淀对血清蛋白进行分级的原始Cohn法。血清白蛋白可在采用Cohn′法的第五乙醇分级中发现。从此，有些人便开始使用“Fraction V”这一术语来描述血清白蛋白，无论使用的是什么制备方法。也有人在使用该术语来描述通过乙醇分级方法而纯化得到血清白蛋白，而这种方法是相对于原始的Cohn法一直在得到高度的改良。Sigma-Aldrich生产和分销从各种主要方法纯化得到的血清白蛋白，包括真正的Cohn分级法、改良的乙醇分级方法、热休克和色谱法。更多的纯化步骤包括结晶或木炭过滤。

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存储类别

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

法规信息

新产品

此项目有

历史批次信息供参考:

分析证书(COA)

Lot/Batch Number

0000420862

0000484379

030M1747