生物来源
goat
偶联物
alkaline phosphatase conjugate
抗体形式
affinity isolated antibody
抗体产品类型
secondary antibodies
克隆
polyclonal
表单
buffered aqueous glycerol solution
技术
direct ELISA: 1:40,000
immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:20
western blot (chemiluminescent): 1:30,000 using β-actin in total cell extract of HiLa cells (5-10 μg per well)
运输
wet ice
储存温度
2-8°C
靶向翻译后修饰
unmodified
正在寻找类似产品? 访问 产品对比指南
一般描述
Mouse IgGs have four distinct isotypes, namely, IgG1, IgG2a, IgG2b, and IgG3. IgG1 regulates complement fixation in mice . Anti-Mouse IgG (Fc specific)-Alkaline Phosphatase antibody is specific for mouse IgG and mouse IgG, Fc fragment. The product does not react with mouse IgG, Fab fragment, human IgG, IgA, and IgM, or rat IgG.
特异性
Anti-Mouse IgG (Fc specific)-Alkaline Phosphatase antibody is specific for mouse IgG and mouse IgG, Fc fragment. The product does not react with mouse IgG, Fab fragment, human IgG, IgA, and IgM, or rat IgG.
免疫原
Purified mouse IgG, Fc fragment
应用
碱性磷酸酶缀合的山羊抗小鼠 Fc 特异性抗体在 ELISA 测定中用作二抗,在 PBS/0.1% Tween 和 1% BSA中以 1:1000 的稀释度,在 37°C 下持续 1.5 小时。该抗体经开发使用磷酸 4-硝基苯酯(Sigma)作为底物,在 37°C 下,持续 30分钟。
其他说明
Antibody adsorbed with human IgG and rat serum proteins.
外形
Solution in 0.05 M Tris, pH 8.0, containing 1% bovine serum albumin, 1 mM MgCl2, 50% glycerol and 15 mM sodium azide.
制备说明
Adsorbed to reduce background staining with human or rat samples.
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
未找到合适的产品?
试试我们的产品选型工具.
储存分类代码
10 - Combustible liquids
WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
个人防护装备
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
历史批次信息供参考:
分析证书(COA)
Lot/Batch Number
我们的科学家团队拥有各种研究领域经验,包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.
联系客户支持