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A8264

4-氨基邻苯二甲酰肼

Name: 4-氨基邻苯二甲酰肼
Brand: SIGMA

≥98% (HPLC), powder

别名:

6-氨基-2,3-二氢-1,4-酞嗪二酮, 异鲁米诺

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关于此项目

经验公式（希尔记法）:

C₈H₇N₃O₂

化学文摘社编号:

3682-14-2

分子量:

177.16

NACRES:

NA.83

PubChem Substance ID:

24891326

UNSPSC Code:

12352204

MDL number:

MFCD00010560

Beilstein/REAXYS Number:

164804

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属性

产品名称

4-氨基邻苯二甲酰肼,

assay

≥98% (HPLC)

Quality Level

200

form

powder

mp

300 °C (lit.)

solubility

2 M NH₄OH: 50 mg/mL, clear to slightly hazy

SMILES string

Nc1ccc2C(=O)NNC(=O)c2c1

InChI

1S/C8H7N3O2/c9-4-1-2-5-6(3-4)8(13)11-10-7(5)12/h1-3H,9H2,(H,10,12)(H,11,13)

InChI key

HUDPLKWXRLNSPC-UHFFFAOYSA-N

说明

Application

4-氨基邻苯二甲酰肼（异鲁米诺）已用于：

假结核耶氏菌感染后骨髓(BM)中性粒细胞的化学发光法活性氧(ROS)检测
克雷伯氏肺炎菌(K. pneumonia)暴露后中性粒细胞的活性氧检测
甲酰-蛋氨酸-亮氨酸-脯氨酸刺激后中性粒细胞的活性氧检测

Biochem/physiol Actions

4-氨基邻苯二甲酰肼或异鲁米诺是由酞嗪环构成的化学发光试剂，亲水性更高于鲁米诺。异鲁米诺在商业上广泛用于各种体外免疫分析，是可用于研究活性氧的放大性化学发光试剂。异鲁米诺也可用作电发光生物传感器(ECL)和微生物快速检测探针。

Features and Benefits

能发出化学荧光。

pictograms

GHS07

signalword

Warning

hcodes

H315,H319,H335

pcodes

P261 - P264 - P271 - P280 - P302 + P352 - P305 + P351 + P338

Hazard Classifications

Eye Irrit. 2 - Skin Irrit. 2 - STOT SE 3

target_organs

Respiratory system

存储类别

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

dust mask type N95 (US), Eyeshields, Gloves

历史批次信息供参考:

分析证书(COA)

Lot/Batch Number

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An attempt to design an isoluminol-hydrogen peroxidase-amplified CL that measures intracellularly produced H2O2 in phagocytes: sensitivity for H2O2 is not high enough to allow detection.

Anna-Lena Stenfeldt et al.

Luminescence : the journal of biological and chemical luminescence, 22(5), 507-510 (2007-07-05)

A technique was designed for the determination of hydrogen peroxide release. We found, however, that the isoluminol-amplified chemiluminescence technique is a suitable tool for measuring secretion of superoxide but not hydrogen peroxide.

A new isoluminol reagent for chemiluminescence labeling of proteins

Palmioli A, et al.

Tetrahedron Letters, 54, 4446-4450 (2013)

Differentiating between intra- and extracellular chemiluminescence in diluted whole-blood samples.

M Rájecký et al.

International journal of laboratory hematology, 34(2), 136-142 (2011-08-13)

The differentiation between extra- and intracellular production of reactive oxygen species (ROS) in whole blood was measured by luminol- and isoluminol-enhanced chemiluminescence (CL). Azide (total CL inhibition), azide + horseradish peroxidase (HRP, restoring extracellular CL), superoxide dismutase + catalase (depleting

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