生物来源
rabbit
质量水平
偶联物
peroxidase conjugate
抗体形式
IgG fraction of antiserum
抗体产品类型
secondary antibodies
克隆
polyclonal
表单
buffered aqueous solution
技术
direct ELISA: 1:30,000
dot blot: 1:2,000
immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:100
western blot (chemiluminescent): 1:160,000
运输
dry ice
储存温度
−20°C
靶向翻译后修饰
unmodified
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一般描述
免疫球蛋白是由两条重链和两条轻链通过二硫键连接而成的糖蛋白。免疫球蛋白g(IgG)是一种主要的免疫球蛋白。山羊IgG 有两个子类:IgG1 和IgG2。然后,使用0.2%戊二醛进行蛋白质交联,使兔抗山羊IgG抗体与过氧化物酶偶联。偶联物为溶液形式,溶于0.01M磷酸盐缓冲盐水,pH 7.4,含有15 mM MIT作为防腐剂。
免疫原
纯化山羊IgG
应用
兔抗山羊IgG(全分子)-过氧化物酶抗体可用于免疫印迹和免疫组织化学。
生化/生理作用
免疫球蛋白主要用于帮助免疫防御。免疫球蛋白G(IgG)参与II型和III型超敏反应。
外形
溶解在0.01M pH7.4磷酸缓冲盐的溶液含有0.05%MIT。
制备说明
通过Avrameas, S.等人在Scand. J. Immunol., 8, Suppl. 7, 7 (1978)中描述的两步戊二醛法制备。
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除非我们的产品目录或产品附带的其他公司文档另有说明,否则我们的产品仅供研究使用,不得用于任何其他目的,包括但不限于未经授权的商业用途、体外诊断用途、离体或体内治疗用途或任何类型的消费或应用于人类或动物。
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