B3148
单克隆抗 山羊/绵羊 IgG
clone GT-34, purified from hybridoma cell culture
别名:
Monoclonal Anti-Goat/Sheep IgG, Monoclonal Anti-Goat/Sheep IgG–Biotin antibody produced in mouse
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关于此项目
NACRES:
NA.46
UNSPSC Code:
12352203
Conjugate:
biotin conjugate
Clone:
GT-34, monoclonal
Application:
ELISA (d)
Citations:
11
biological source
mouse
conjugate
biotin conjugate
antibody form
purified from hybridoma cell culture
antibody product type
secondary antibodies
clone
GT-34, monoclonal
form
buffered aqueous solution
species reactivity
sheep, goat, bovine
should not react with
rat, canine, mouse, rabbit, guinea pig, chicken, horse, pig, feline, human
technique(s)
direct ELISA: 1:5,000
isotype
IgG1
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Quality Level
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General description
抗体与位于山羊 IgG1 和 IgG2 重链上的表位反应。
Monoclonal Anti-Goat IgG (mouse IgG1 isotype) is derived from the hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse.
The product binds to all goat/sheep IgG1
Application
Monoclonal Anti-Goat/Sheep IgG-Biotin antibody produced in mouse has been used in:
- enzyme linked immuno sorbent assay (ELISA)
- immunoblotting
- dot blotting
- immunohistochemistry
- immunocytochemistry
Monoclonal Anti-Goat/Sheep IgG-Biotin antibody produced in mouse may be used for ELISA at a minimum working dilution of 1:5000. It was used at a working dilution of 1:1000 to visualize collagen type IV in mouse digit sections.
Biochem/physiol Actions
IgG antibody subtype is the most abundant of serum immunoglobulins of the immune system. It is secreted by B cells and is found in blood and extracellular fluids and provides protection from infections caused by bacteria, fungi and viruses. Maternal IgG is transferred to fetus through the placenta that is vital for immune defense of the neonate against infections. The coupling of biotin allows for the binding of various labels such as avidin or streptavidin.
Physical form
Solution in 0.01 M phosphate buffered saline pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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存储类别
12 - Non Combustible Liquids
wgk
nwg
flash_point_f
Not applicable
flash_point_c
Not applicable
法规信息
常规特殊物品
低风险生物材料
此项目有
L Li et al.
Scandinavian journal of immunology, 57(5), 410-422 (2003-05-20)
Involution of the thymus and alterations in the development of thymocytes are the most prominent features of age-related immune senescence. We have carried out a comparative analysis of thymocyte and stroma in rapid thymic involution DBA/2 (D2) strain of mice
Dynamic changes after murine digit amputation: the MRL mouse digit shows waves of tissue remodeling, growth, and apoptosis
Gourevitch DL, et al.
Wound Repair and Regeneration, 17(3), 447 -4455 (2009)
Sofia Halin Bergström et al.
Scientific reports, 6, 31805-31805 (2016-08-24)
Accumulating data indicates that tumor-derived extracellular vesicles (EVs) are responsible for tumor-promoting effects. However, if tumor EVs also prepare the tumor-bearing organ for subsequent tumor growth, and if this effect is different in low and high malignant tumors is not
Lara Sánchez-Trujillo et al.
Journal of personalized medicine, 13(6) (2023-06-28)
Chronic venous disease (CVD) is a common condition that affects the veins in the lower limbs, resulting in a variety of symptoms, such as swelling, pain, and varicose veins (VVs). The plenty hormonal, hemodynamic and mechanical changes occurred in pregnancy
Oscar Fraile-Martinez et al.
Histology and histopathology, 39(1), 35-40 (2023-04-15)
Pancreatic cancer is a malignant neoplasm that, despite its low frequency, has a 5-year survival rate of less than 10%. The study of different histopathological markers has allowed a better understanding of the onset and development of this type of
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