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Merck
CN

B9146

5-溴-4-氯-3-吲哚基-β-吡咯-D-半乳糖苷

≥98%, powder, suitable for identification of lac+ bacterial colonies

别名:

5-溴-4-氯-3-吲哚基-β-吡咯-D-半乳糖苷, BCIG, X-Gal

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关于此项目

经验公式(希尔记法):
C14H15BrClNO6
化学文摘社编号:
分子量:
408.63
NACRES:
NA.83
PubChem Substance ID:
UNSPSC Code:
12352204
EC Number:
230-640-8
MDL number:
Beilstein/REAXYS Number:
1402009
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产品名称

5-溴-4-氯-3-吲哚基-β-吡咯-D-半乳糖苷, ≥98%

Quality Level

assay

≥98%

form

powder

solubility

DMF: methanol (1:1): soluble 50 mg/mL

suitability

suitable for identification of lac+ bacterial colonies

storage temp.

−20°C

SMILES string

OC[C@H]1O[C@@H](Oc2c[nH]c3ccc(Br)c(Cl)c23)[C@H](O)[C@@H](O)[C@H]1O

InChI

1S/C14H15BrClNO6/c15-5-1-2-6-9(10(5)16)7(3-17-6)22-14-13(21)12(20)11(19)8(4-18)23-14/h1-3,8,11-14,17-21H,4H2/t8-,11+,12+,13-,14-/m1/s1

InChI key

OPIFSICVWOWJMJ-AEOCFKNESA-N

Application

5-溴-4-氯-3-吲哚 β-D-半乳吡喃糖苷已被用于验证Caki-2/LacZ克隆的转导是否成功。它还可用于转化子的蓝白班筛选。
X-Gal是β-半乳糖苷酶的发色底物,可产生丰富的蓝色,与背景相比可以直观地检测到。 X-Gal是lac+基因型重组细菌菌落蓝白斑筛选的首选基质。

Preparation Note

储备溶液(100 mg/ml)是通过将 X-Gal 溶解在二甲基甲酰胺中制备的,该溶液应避光储存在 −20 °C。无需灭菌。


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存储类别

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

法规信息

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实验方案

Yeasts are considered model systems for eukaryotic studies as they exhibit fast growth and have dispersed cells.

Technical Article on competent cells. Transformation is a process by which some bacteria take up foreign genetic material (naked DNA) from the environment.

General protocols for growth of competent cells in microbial medium.

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商品

General protocols for growth of competent cells and their transformation (uptake of DNA).

Transformation introduces exogenous DNA into cells, a fundamental genetic modification process demonstrated in Streptococcus pneumoniae.

感受态细胞生长及其转化(吸收DNA)的通用实验方案。

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HSV-tk gene therapy for human renal cell carcinoma in nude mice
Pulkkanen K, et al.
Cancer Gene Therapy, 8(7), 529-529 (2001)
Rolling circle amplification to screen yam germplasm for badnavirus infections and to amplify and characterise novel badnavirus genomes
Bomer M, et al.
Bio-protocol, 8(1), 529-529 (2018)
Yibing Wang et al.
PloS one, 8(3), e59195-e59195 (2013-03-26)
Our study had three objectives: to extend the plasmid-based transformation protocol to a clinical isolate of C. trachomatis belonging to the trachoma biovar, to provide "proof of principle" that it is possible to "knock out" selected plasmid genes (retaining a



全球贸易项目编号

货号GTIN
B9146-100MG04061835553976
B9146-10MG04061835540716
B9146-500MG04061835511846