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主要文件

BRIN-BG7

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA.

别名:

BRIN

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About This Item

UNSPSC代码:
41106514

生物来源

rat pancreas (islets)

包装

tube of 5 μg 10020902-DNA-5UG
pkg of vial of cells 10020902-1VL

生长模式

Adherent

核型

Modal chromosome number 66 up to passage 50

形态学

Epitheloid

产品

BRIN-BG7 is a stable, glucose-responsive insulin secreting beta cell line expressing insulin, glucokinase and IAPP as detected by immunocytochemistry. BRIN-BG7 cells express the GLUT2 glucose transporter.

受体

Not specified

技术

cell culture | mammalian: suitable

运输

dry ice

细胞系来源

A rat pancreatic Beta cell: RINm5F hybrid cell line. Insulin-secreting cell line

DNA图谱分析

Not specified

培养基

RPMI-1640 + 2 mM Glutamine + 10% FCS / FBS

传代培养常规

Split sub-confluent cultures (70-80%) 1:8 to 1:10 , i.e., seeding at 2-4 x 104 cells/cm2 using 0.05% trypsin or trypsin/EDTA; 5% CO2; 37 °C. Population doubling approx 20hrs. At confluence 105 cells/cm2 can be expected.

其他说明

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

免责声明

This cell line has special release conditions: Commercial organisations are required to complete the ′Cell Line Release Authorisation for Research Use in Commercial Organisations′ release conditions form.

历史批次信息供参考:

分析证书(COA)

Lot/Batch Number

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