生物来源
hamster kidney
包装
tube of 5 μg 84111301-DNA-5UG
pkg of vial of cells 84111301-1VL
生长模式
Suspension
核型
2n = 44, pseudodiploid
形态学
Not specified
产品
Not specified
受体
Not specified
技术
cell culture | mammalian: suitable
运输
dry ice
储存温度
−196°C
相关类别
细胞系来源
Hamster Syrian kidney
细胞系描述
Derived from the parent line BHK-21 (Clone-13) (Sigma Catalogue number. 85011433). The cells have been adapted to growth in suspension and have the same virus susceptibility as the parent line.
应用
Virus replication studies
培养基
GMEM + 2mM Glutamine + 5% Tryptose Phosphate Broth + 10% Foetal Bovine Serum (FBS).
传代培养常规
Maintain cultures between 2-9x100,000 cells/ml (split ratio of 1:3 to 1:6); 5% CO2; 37°C. Cells need to be grown in a techni-stirrer or they may start to re-attach.
其他说明
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