biological source
rat kidney
packaging
tube of 5 μg 86101301-DNA-5UG, pkg of vial of cells 86101301-1VL
growth mode
Adherent
karyotype
Not specified
morphology
Fibroblast
products
Not specified
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
shipped in
dry ice
storage temp.
−196°C
Application
Contact inhibition and transformation studies
Biochem/physiol Actions
Derived from normal kidney and exhibits contact-inhibition. It is sensitive to viral or chemical transformation, including proteins such as SGF. Originated from the same mixed culture as NRK-52E but has distinct characteristics.
Rat kidney fibroblast
Preparation Note
DMEM + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 5-10% Foetal Bovine Serum (FBS) or New Born Calf Serum (NBCS).
Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C. The cells should be maintained sub-confluent, otherwise they will transform.
Other Notes
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法规信息
低风险生物材料
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