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RPMI 8226

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, 87012702, human blood, Not specified

别名:

RPMI8226 Cells, Roswell Park Memorial Institute 8226 Cells

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关于此项目

UNSPSC Code:
41106514
Biological source:
human blood
Relevant disease(s):
cancer
Growth mode:
Suspension
Karyotype:
Not specified
Morphology:
Not specified
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biological source

human blood

packaging

tube of 5 μg 87012702-DNA-5UG, pkg of vial of cells 87012702-1VL

growth mode

Suspension

karyotype

Not specified

morphology

Not specified

products

Immunoglobulin G (Ig) lambda light chain

receptors

Not specified

technique(s)

cell culture | mammalian: suitable

relevant disease(s)

cancer

shipped in

dry ice

storage temp.

−196°C

Application

RPMI 8226 has been used:
  • to grow human myeloma cell lines
  • to evaluate the effect of 1-aryl-2-methylthioimidazolines (IIV) on the viability of RPMI 8226 cells
  • to measure cytokine production by bone marrow stromal cells (BMSCs) after contact with the RPMI 8226 cell line

Biochem/physiol Actions

Derived from the peripheral blood of a 61 year old male with multiple myeloma. The cells produce and secrete Ig lambda light chain.
Human myeloma
STR-PCR Data: Amelogenin: X,Y
CSF1PO: 12
D13S317: 11
D16S539: 9
D5S818: 11,13
D7S820: 9,10
THO1: 8
TPOX: 8,11
vWA: 16,18

Preparation Note

Maintain cultures between 3-9x100,000 cells/ml; 5% CO2; 37°C. After resuscitation cell debris will be present in culture. This will decrease with subsequent subculture.
RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Other Notes

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

法规信息

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