biological source
human blood
packaging
tube of 5 μg 87012702-DNA-5UG, pkg of vial of cells 87012702-1VL
growth mode
Suspension
karyotype
Not specified
morphology
Not specified
products
Immunoglobulin G (Ig) lambda light chain
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
relevant disease(s)
cancer
shipped in
dry ice
storage temp.
−196°C
Application
RPMI 8226 has been used:
- to grow human myeloma cell lines
- to evaluate the effect of 1-aryl-2-methylthioimidazolines (IIV) on the viability of RPMI 8226 cells
- to measure cytokine production by bone marrow stromal cells (BMSCs) after contact with the RPMI 8226 cell line
Biochem/physiol Actions
Derived from the peripheral blood of a 61 year old male with multiple myeloma. The cells produce and secrete Ig lambda light chain.
Human myeloma
STR-PCR Data: Amelogenin: X,Y
CSF1PO: 12
D13S317: 11
D16S539: 9
D5S818: 11,13
D7S820: 9,10
THO1: 8
TPOX: 8,11
vWA: 16,18
CSF1PO: 12
D13S317: 11
D16S539: 9
D5S818: 11,13
D7S820: 9,10
THO1: 8
TPOX: 8,11
vWA: 16,18
Preparation Note
Maintain cultures between 3-9x100,000 cells/ml; 5% CO2; 37°C. After resuscitation cell debris will be present in culture. This will decrease with subsequent subculture.
RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).
Other Notes
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法规信息
常规特殊物品
低风险生物材料
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