RPMI 8226
NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, 87012702, human blood, Not specified
别名:
RPMI8226 Cells, Roswell Park Memorial Institute 8226 Cells
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About This Item
UNSPSC代码:
41106514
生物来源
human blood
包装
tube of 5 μg 87012702-DNA-5UG
pkg of vial of cells 87012702-1VL
生长模式
Suspension
核型
Not specified
形态学
Not specified
产品
Immunoglobulin G (Ig) lambda light chain
受体
Not specified
技术
cell culture | mammalian: suitable
相关疾病
cancer
运输
dry ice
储存温度
−196°C
相关类别
细胞系来源
Human myeloma
细胞系描述
Derived from the peripheral blood of a 61 year old male with multiple myeloma. The cells produce and secrete Ig lambda light chain.
应用
RPMI 8226 has been used:
- to grow human myeloma cell lines
- to evaluate the effect of 1-aryl-2-methylthioimidazolines (IIV) on the viability of RPMI 8226 cells
- to measure cytokine production by bone marrow stromal cells (BMSCs) after contact with the RPMI 8226 cell line
DNA图谱分析
STR-PCR Data: Amelogenin: X,Y
CSF1PO: 12
D13S317: 11
D16S539: 9
D5S818: 11,13
D7S820: 9,10
THO1: 8
TPOX: 8,11
vWA: 16,18
CSF1PO: 12
D13S317: 11
D16S539: 9
D5S818: 11,13
D7S820: 9,10
THO1: 8
TPOX: 8,11
vWA: 16,18
培养基
RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).
传代培养常规
Maintain cultures between 3-9x100,000 cells/ml; 5% CO2; 37°C. After resuscitation cell debris will be present in culture. This will decrease with subsequent subculture.
其他说明
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