生物来源
mink lung
包装
tube of 5 μg 88050503-DNA-5UG
pkg of vial of cells 88050503-1VL
生长模式
Adherent
核型
2n = 30; diploid and pseudodiploid
形态学
Epithelial
产品
Not specified
受体
Not specified
技术
cell culture | mammalian: suitable
运输
dry ice
储存温度
−196°C
细胞系来源
Mink lung
细胞系描述
Derived from trypsinised lungs of several foetuses of Aleutian mink. Used for focus forming assays for murine and feline sarcoma viruses.
应用
Virus focus forming assays: MSV, feline sarcoma virus
培养基
EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).
传代培养常规
Split sub-confluent cultures (70-80%) 1:2 to 1:4 i.e. seeding at 2-4x10,000 cells/cm2 using 0.25% trypsin/EDTA; 5% CO2; 37°C.
其他说明
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