生物来源
mouse nerve
包装
tube of 5 μg 89121405-DNA-5UG
pkg of vial of cells 89121405-1VL
生长模式
Adherent
核型
Unstable
形态学
Neuroblast
产品
Acetylcholinesterase, choline acetylase, tyrosine hydroxylase
受体
Not specified
技术
cell culture | mammalian: suitable
相关疾病
cancer
运输
dry ice
储存温度
−196°C
相关类别
细胞系来源
Mouse C-1300 Neuroblastoma
细胞系描述
Cloned from the C1300 mouse neuroblastoma line. Adapted to culture by alternate in vivo and in vitro passage. Cells have characteristics of mature neurons and secrete the enzymes for the synthesis of the neurotransmitters, choline acetylase and tyrosine hydroxylase. Cells are susceptible to VSV, HSV and Vaccinia viruses.
应用
Tumourigenicity, enzymatic and virus studies: VSV, HSV, vaccinia virus
培养基
Ham′s F10 + 2mM Glutamine + 15% Horse Serum (HS) + 2.5% Foetal Bovine Serum (FBS).
传代培养常规
Split sub-confluent cultures (70-80%) 1:2 to 1:4 i.e. seeding at 3-5x10,000 cells/cm2 using 0.25% trypsin; 5% CO2; 37°C.
其他说明
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