biological source
mouse blood
packaging
tube of 5 μg 92021401-DNA-5UG, pkg of vial of cells 92021401-1VL
growth mode
Suspension
karyotype
Not specified
morphology
Lymphoblast
products
Not specified
receptors
Interleukin 2 (IL-2), Interleukin 4 (IL-4)
technique(s)
cell culture | mammalian: suitable
shipped in
dry ice
storage temp.
−196°C
Application
IL-2 assays
Biochem/physiol Actions
Derived in 1979 from BALB/c spleen cells activated by sheep erythrocytes in the presence of IL-2, the A5E clone was selected to provide a homogenous population that is highly sensitive to IL-2. This cell line expresses Thy-1 and Lyt markers in addition to high affinity IL-2 receptors. Substantial proliferation is seen in the presence of IL-4 (GM-CSF).
Mouse helper T cell
Preparation Note
Maintain cultures between 3-9x100,000 cells/ml; 5% CO2; 37°C. When starting from a frozen ampoule, add thawed cells to a conical based centrifuge tube e.g. 15ml tube. Slowly add 4 ml of culture medium to the tube. Take a sample of the cell suspension, e.g
RPMI 1640 + 2mM Glutamine + 0.05mM 2-Mercaptoethanol (2ME) + 100-200 IU/ml IL-2 + 10% Foetal Bovine Serum (FBS).
Other Notes
Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.
法规信息
低风险生物材料
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