生物来源
human blood
包装
tube of 5 μg 92110206-DNA-5UG
pkg of vial of cells 92110206-1VL
生长模式
Suspension
核型
Not specified
形态学
Lymphoblast
产品
Surface Immunoglobulin ( Ig), MHC Class II
受体
Fc, complement
技术
cell culture | mammalian: suitable
相关疾病
cancer
运输
dry ice
储存温度
−196°C
相关类别
细胞系来源
Human lymphoblast
细胞系描述
Derived from a patient with myelomonocytic leukaemia and subsequently exposed to Epstein Barr Virus (EBV). This line is EBV positive and therefore presumably derived from a normal B lymphocyte. This cell line is also positive for surface Ig, Fc receptor, complement receptor, surface Ia and EBNA. Responds to T cell replacement factor by increasing the number of IgG secreting cells. The cells should be handled under laboratory containment level 2.
应用
T cell replacement factor (TRF) assays, B cell differentiation studies
DNA图谱分析
STR-PCR Data: Amelogenin: X,Y
CSF1PO: 10,11
D13S317: 12
D16S539: 12
D5S818: 11,12
D7S820: 10,12
THO1: 7,9.3
TPOX: 8,9
vWA: 16,17
CSF1PO: 10,11
D13S317: 12
D16S539: 12
D5S818: 11,12
D7S820: 10,12
THO1: 7,9.3
TPOX: 8,9
vWA: 16,17
培养基
RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).
传代培养常规
Maintain cultures between 2-9x100,000 cells/ml; 5% CO2; 37°C.
其他说明
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